ARGONAUTE 2 increases rice susceptibility to rice black-streaked dwarf virus infection by epigenetically regulating HEXOKINASE 1 expression,Molecular Plant Pathology

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ARGONAUTE 2 increases rice susceptibility to rice black-streaked dwarf virus infection by epigenetically regulating HEXOKINASE 1 expression
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-06-10 , DOI: 10.1111/mpp.13091
Zhaoyun Wang ₁ , Dongyue Chen _{1,

2} , Feng Sun ₁ , Wei Guo _{1,

3} , Wei Wang _{1,

2} , Xuejuan Li ₁ , Ying Lan ₁ , Linlin Du ₁ , Shuo Li ₁ , Yongjian Fan ₁ , Yijun Zhou ₁ , Hongwei Zhao ₂ , Tong Zhou _{1,

4}

Affiliation

ARGONAUTE (AGO) proteins play crucial roles in plant defence against virus invasion. To date, the role of OsAGO2 in rice antiviral defence remains largely unknown. In this study, we determined that the expression of OsAGO2 in rice was induced upon rice black-streaked dwarf virus (RBSDV) infection. Using transgenic rice plants overexpressing OsAGO2 and Osago2 mutants generated through transposon-insertion or CRISPR/Cas9 technology, we found that overexpression of OsAGO2 enhanced rice susceptibility to RBSDV infection. Osago2 mutant lines exhibited strong resistance to RBSDV infection through the elicitation of an early defence response, including reprogramming defence gene expression and production of reactive oxygen species (ROS). Compared to Nipponbare control, the expression level of OsHXK1 (HEXOKINASE 1) increased significantly, and the methylation levels of its promoter decreased in the Osago2 mutant on RBSDV infection. The expression profile of OsHXK1 was the opposite to that of OsAGO2 during RBSDV infection. Overexpression of OsHXK1 in rice also induced ROS production and enhanced rice resistance to RBSDV infection. These results indicate that OsHXK1 controls ROS accumulation and is regulated by OsAGO2 through epigenetic regulation. It is noteworthy that the Osago2 mutant plants are also resistant to southern rice black-streaked dwarf virus infection, another member of the genus Fijivirus. Based on the results presented in this paper, we conclude that OsAGO2 modulates rice susceptibility to fijivirus infection by suppressing OsHXK1 expression, leading to the onset of ROS-mediated resistance. This discovery may benefit future rice breeding programmes for virus resistance.

中文翻译：

ARGONAUTE 2 通过表观遗传调控 HEXOKINASE 1 表达增加水稻对水稻黑条矮缩病毒感染的易感性

ARGONAUTE (AGO) 蛋白在植物防御病毒入侵中起着至关重要的作用。迄今为止，OsAGO2在水稻抗病毒防御中的作用仍然很大程度上未知。在本研究中，我们确定水稻黑条矮缩病毒 (RBSDV) 感染可诱导水稻中OsAGO2的表达。使用过表达OsAGO2和通过转座子插入或 CRISPR/Cas9 技术产生的Osago2突变体的转基因水稻植物，我们发现OsAGO2的过表达增强了水稻对 RBSDV 感染的易感性。Osago2突变株系通过引发早期防御反应表现出对 RBSDV 感染的强烈抵抗力，包括重新编程防御基因表达和活性氧 (ROS) 的产生。与日本晴对照相比， RBSDV感染后Osago2突变体中OsHXK1 ( HEXOKINASE 1 )的表达水平显着升高，其启动子甲基化水平降低。在 RBSDV 感染期间， OsHXK1的表达谱与OsAGO2的表达谱相反。OsHXK1在水稻中的过表达也诱导了 ROS 的产生并增强了水稻对 RBSDV 感染的抗性。这些结果表明OsHXK1控制 ROS 积累并受OsAGO2通过表观遗传调控。值得注意的是，Osago2突变植物也能抵抗斐济病毒属的另一个成员南方水稻黑条矮缩病毒感染。基于本文提供的结果，我们得出结论，OsAGO2通过抑制OsHXK1的表达来调节水稻对斐济病毒感染的易感性，从而导致 ROS 介导的抗性的发生。这一发现可能有利于未来的水稻抗病毒育种计划。

更新日期：2021-08-12

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