Brucellosis is a zoonotic disease caused by a Gram-negative coccobacillus. There are four Brucella strains of zoonotic importance in our domestic species, subdivided by their culture phenotypes: Brucella abortus (B. abortus), B. melitensis, B. suis (smooth strains) and B. canis (rough strain). Dogs can serve as hosts for all four of the zoonotic strains; however, routine serologic testing in dogs has been limited to the identification of B. canis antibodies. The aim of our study was to identify smooth Brucella strain antibodies in canines. We hypothesize that the Brucella abortus Fluorescence Polarization Assay would be successful in identifying smooth Brucella strain antibodies in canines. Ninety-five dogs, including forty-five hog hunting dogs were screened for circulating antibodies to any of the four zoonotic strains of the bacteria utilizing a combination of Canine Brucella Slide Agglutination Test (CBSA), Brucella canis Agar Gel Immunodiffusion II test (AGID), Brucella abortus Card Agglutination Test (BCA), and the Brucella abortus Fluorescence Polarization Assay (FPA). Test interpretation results yielded a 0% (0/95) smooth Brucella strain seropositivity rate, with 2% (2/95) of dogs yielding inconclusive rough Brucella strain serology results (0-2% rough strain seropositivity rate). Additionally, a retrospective portion of the study was performed to identify sera containing circulating antibodies to any of the smooth strains of Brucella by testing previously banked canine serum samples stored at Cornell’s Veterinary Diagnostic Laboratory from 2018-2019 via Brucella abortus FPA. Of the 769 serum samples tested, 13/769 (1.7%) yielded an inconclusive result, 725/769 (94.2%) were negative, 30/769 (4%) yielded a positive FPA test result, and 1/769 (0.1%) had to be excluded due to insufficient sample remaining to perform the diagnostic test. Of the 30 FPA positive canine serum samples, 97% (29/30) also tested positive on the CBSA test. Additionally, there was a statistically significant (p-value: <0.0001) likelihood of altered (spayed/neutered) and mixed breed dogs to be FPA positive when compared to intact, purebred dogs respectively.

中文翻译：

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犬平滑布鲁氏菌抗体的推定鉴定

布鲁氏菌病是一种由革兰氏阴性球杆菌引起的人畜共患疾病。在我们的家养物种中有四种具有人畜共患重要性的布鲁氏菌菌株，按其培养表型细分：流产布鲁氏菌（B. abortus）、羊种布鲁氏菌、猪布鲁氏菌（平滑菌株）和犬布鲁氏菌（粗糙菌株）。狗可以作为所有四种人畜共患菌株的宿主；然而，狗的常规血清学检测仅限于鉴定犬双歧杆菌抗体。我们研究的目的是在犬科动物中鉴定平滑布鲁氏菌菌株抗体。我们假设流产布鲁氏菌荧光偏振分析将成功鉴定犬科动物中的平滑布鲁氏菌菌株抗体。九十五条狗，使用犬布鲁氏菌玻片凝集试验 (CBSA)、犬布鲁氏菌琼脂凝胶免疫扩散 II 试验 (AGID)、流产布鲁氏菌卡片凝集试验的组合，对包括 45 只猎猪犬在内的四种人畜共患细菌菌株中的任何一种进行循环抗体筛查测试 (BCA) 和布鲁氏菌流产荧光偏振分析 (FPA)。测试解释结果产生 0% (0/95) 平滑布鲁氏菌菌株血清阳性率，2% (2/95) 狗产生不确定的粗糙布鲁氏菌菌株血清学结果（0-2% 粗糙菌株血清阳性率）。此外，该研究的回顾性部分是通过检测 2018 年至 2019 年间通过布鲁氏菌流产 FPA 储存在康奈尔大学兽医诊断实验室的先前储存的犬血清样本来鉴定含有针对任何布鲁氏菌平滑菌株的循环抗体的血清。在测试的 769 个血清样本中，13/769 (1.7%) 的结果不确定，725/769 (94.2%) 为阴性，30/769 (4%) 的 FPA 测试结果为阳性，1/769 (0.1%) ) 由于剩余样本不足而无法进行诊断测试，因此必须排除在外。在 30 个 FPA 阳性犬血清样本中，97% (29/30) 在 CBSA 测试中也呈阳性。此外，与完整的纯种狗相比，改变（绝育/绝育）和混合品种狗为 FPA 阳性的可能性分别具有统计学意义（p 值：<0.0001）。