Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to pain, discomfort, and unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app for doses calculation and a standard dose calculation. A total 939 C57BL/6J and Swiss mice were analyzed. We collected intraoperative and anesthesia-related mortality described in electronic or physical handwritten records. Our results showed that the mobile app approach significantly reduces anesthetic-related deaths upon using doses of ketamine and xylazine. The results suggest that anesthesia related mortality can be minimized even more using information technology approaches, helping to solve an old but transversal challenge for researchers working with experimental mice. The mobile app is a free and open code which could be implemented worldwide as an essential requirement for all anesthetic procedures in mice using xylazine and ketamine combination. Also, as an open code app, Labinsane initiative could represent the starting point for unify and validate other anesthetic procedures in different species and strains.

中文翻译：

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用于个体甲苯噻嗪/氯胺酮计算的智能手机应用程序降低了小鼠的麻醉相关死亡率

目前，实验动物被广泛用于生物学和医学研究。然而，科学界提出了一些生物伦理问题，例如实现可重复和统计相关结果所需的动物数量。这些问题涉及与疼痛、不适和不必要的动物损失相关的方面。回顾起来，我们比较了两种不同的麻醉剂量方法：用于剂量计算的移动应用程序和标准剂量计算。总共分析了 939 只 C57BL/6J 和 Swiss 小鼠。我们收集了电子或物理手写记录中描述的术中和麻醉相关死亡率。我们的结果表明，移动应用程序方法可显着减少使用氯胺酮和甲苯噻嗪剂量后与麻醉剂相关的死亡。结果表明，使用信息技术方法可以进一步降低与麻醉相关的死亡率，这有助于解决研究人员对实验小鼠的一项古老但横向的挑战。该移动应用程序是一个免费和开放的代码，可以在全球范围内实施，作为使用甲苯噻嗪和氯胺酮组合的小鼠所有麻醉程序的基本要求。此外，作为一个开放代码应用程序，Labinsane 计划可以代表统一和验证不同物种和菌株的其他麻醉程序的起点。该移动应用程序是一个免费和开放的代码，可以在全球范围内实施，作为使用甲苯噻嗪和氯胺酮组合的小鼠所有麻醉程序的基本要求。此外，作为一个开放代码应用程序，Labinsane 计划可以代表统一和验证不同物种和菌株的其他麻醉程序的起点。该移动应用程序是一个免费和开放的代码，可以在全球范围内实施，作为使用甲苯噻嗪和氯胺酮组合的小鼠所有麻醉程序的基本要求。此外，作为一个开放代码应用程序，Labinsane 计划可以代表统一和验证不同物种和菌株的其他麻醉程序的起点。