Background

Echinococcosis is a common health problem in the Mediterranean and the Middle East, and manifests without any symptoms, even in the advanced stages.

Objective

The present study aimed to investigate the cell mediated-immunoregulatory milieu in rats’ echinococcosis induced by three different viability status of Echinococcus granulosus especially in the semi-calcareous stage, which can be used as novel biomarkers to monitor disease progression and open the door to a deeper understanding of the pathways that could contribute to complementary echinococcosis therapies.

Materials and Methods

Rat infection with echinococcosis was induced by three different viable statuses of Echinococcus granulosus (G6) camel strain. During the different stages of parasitic infection, blood serum was harvested from rats containing low-, high-, and not viable (not completely transformed to the calcareous status) protoscoleces fluid. The host Th1/Th2 cytokines-mediated immune cell activation, as well as CD3/TCRE immunoregulation, and proliferation responses were investigated; especially in the semi-calcareous stage as this is the first report characterizing this stage.

Results

Both IFN-γ and IL-6 levels significantly increased in the infected groups (P < 0.05), in addition, increased positive immunoreactions in splenic tissue for both CD3/TCRE and Ki-67 monoclonal antibodies.

Conclusion

E. granuloses infection-induced immune tolerance is involved in disease progression, and modulates the activation and regulation of host immune response, even in the early stages of infection, rather than the last stages of viability (semi-calcareous) is not neglected stage. This study is the first to report that the semi-calcareous stage causes a severe immunological response.

中文翻译：

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不同阶段包虫囊肿在二级中间宿主中诱导CD3/TCRE表达和免疫调节环境

背景

棘球蚴病是地中海和中东地区常见的健康问题，即使在晚期阶段也没有任何症状。

客观的

本研究旨在探讨三种不同的细粒棘球蚴病诱导大鼠棘球蚴病的细胞介导免疫调节环境，特别是在半钙质阶段，该研究可作为新的生物标志物监测疾病进展并打开大门。更深入地了解可能有助于补充包虫病治疗的途径。

材料和方法

大鼠感染棘球蚴病是由细粒棘球绦虫(G6) 骆驼株的三种不同存活状态诱导的。在寄生虫感染的不同阶段，从含有低、高和不可行（未完全转化为钙质状态）原节节液的大鼠身上采集血清。研究了宿主 Th1/Th2 细胞因子介导的免疫细胞活化，以及 CD3/TCRE 免疫调节和增殖反应；尤其是在半钙质阶段，因为这是该阶段的第一份报告。

结果

感染组的 IFN-γ 和 IL-6 水平均显着升高（P  < 0.05），此外，CD3/TCRE 和 Ki-67 单克隆抗体在脾组织中的阳性免疫反应增加。

结论

E。 _ 颗粒物感染诱导的免疫耐受参与疾病进展，并调节宿主免疫反应的激活和调节，即使在感染的早期阶段，而不是存活的最后阶段（半钙质）也不被忽视。这项研究首次报道了半钙质阶段会导致严重的免疫反应。