2,4,6-trinitrotoluene (TNT) and cobalt (Co) contaminants have posed a severe environmental problem in many countries. Phytoremediation is an environmentally friendly technology for the remediation of these contaminants. However, the toxicity of TNT and cobalt limit the efficacy of phytoremediation application. The present research showed that expressing the Acidithiobacillus ferrooxidans single-strand DNA-binding protein gene (AfSSB) can improve the tolerance of Arabidopsis and tall fescue to TNT and cobalt. Compared to control plants, the AfSSB transformed Arabidopsis and tall fescue exhibited enhanced phytoremediation of TNT and cobalt separately contaminated soil and co-contaminated soil. The comet analysis revealed that the AfSSB transformed Arabidopsis suffer reduced DNA damage than control plants under TNT or cobalt exposure. In addition, the proteomic analysis revealed that AfSSB improves TNT and cobalt tolerance by strengthening the reactive superoxide (ROS) scavenging system and the detoxification system. Results presented here serve as strong theoretical support for the phytoremediation potential of organic and metal pollutants mediated by single-strand DNA-binding protein genes.

Summarizes

This is the first report that AfSSB enhances phytoremediation of 2,4,6-trinitrotoluene and cobalt separately contaminated and co-contaminated soil.

中文翻译：

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AfSSB转化植物对 TNT 和钴共污染土壤的增强植物修复

2,4,6-三硝基甲苯 (TNT) 和钴 (Co) 污染物已在许多国家造成严重的环境问题。植物修复是一种修复这些污染物的环保技术。然而，TNT 和钴的毒性限制了植物修复应用的功效。目前的研究表明，表达氧化亚铁酸硫杆菌单链DNA结合蛋白基因（AfSSB）可以提高拟南芥和高羊茅对TNT和钴的耐受性。与对照植物相比，AfSSB转化的拟南芥和高羊茅表现出增强的 TNT 和钴分别污染土壤和共污染土壤的植物修复。彗星分析表明，AfSSB在 TNT 或钴暴露下，转化拟南芥的 DNA 损伤比对照植物减少。此外，蛋白质组学分析表明，AfSSB通过加强活性超氧化物 (ROS) 清除系统和解毒系统来提高 TNT 和钴耐受性。这里呈现的结果为单链 DNA 结合蛋白基因介导的有机和金属污染物的植物修复潜力提供了强有力的理论支持。

总结

这是AfSSB增强 2,4,6-三硝基甲苯和钴分别污染和共污染土壤的植物修复的第一份报告。