Combined Strategies to Boost Polyhydroxyalkanoate Production from Fruit Waste in a Three-Stage Pilot Plant,ACS Sustainable Chemistry & Engineering

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Combined Strategies to Boost Polyhydroxyalkanoate Production from Fruit Waste in a Three-Stage Pilot Plant
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2021-06-09 , DOI: 10.1021/acssuschemeng.1c02432
Mariana Matos ₁ , Rafaela A. P. Cruz ₁ , Pedro Cardoso ₁ , Fernando Silva ₁ , Elisabete B. Freitas ₁ , Gilda Carvalho ₁ , Maria A. M. Reis ₁

Affiliation

The full-scale implementation of polyhydroxyalkanoate (PHA) production using mixed microbial cultures (MMCs) has been limited by the low PHA global productivity and overall process yield. This work aimed to demonstrate, at a pilot scale, that by combining different effective operating conditions, it is possible to boost the PHA production performance when using fruit waste as a substrate. The organic loading rate (OLR) and pH of the acidogenic reactor were successfully used as tuning parameters to obtain a high fermentation yield (0.74 gCOD·gCOD^–1) and a fermentate rich in butyrate, resulting in enhanced PHA production steps. A biomass highly enriched in PHA-storing microorganisms was selected as a result of uncoupling the carbon to the nitrogen feeding. The biomass concentration attained a notable value (7.83 g·L^–1) as a response to the high OLR (8.7 gCOD·L^–1·d^–1) imposed. In the PHA accumulation assays, the culture selected at the optimal OLR in the selection reactor achieved a high storage yield (0.98 gCOD·gCOD^–1), and the continuous feeding strategy led to a maximum PHA content of 80.5% (g-basis). The high global productivity (8.1 g-PHA·L^–1·d^–1) and overall process yield (0.45 gCOD·gCOD^–1) are, to the best of the authors’ knowledge, the highest values reported for MMC using a real feedstock at pilot scale. These results demonstrate the importance of combining different effective strategies to maximize the process performance, a promising result toward the full-scale implementation of PHA production from wastes and MMC.

中文翻译：

在三阶段试验工厂中促进从水果废料中生产聚羟基链烷酸酯的综合策略

使用混合微生物培养物 (MMC) 生产聚羟基链烷酸酯 (PHA) 的全面实施受到 PHA 全球生产率和整体工艺产量低的限制。这项工作旨在以中试规模证明，通过结合不同的有效操作条件，可以在使用水果废料作为基质时提高 PHA 的生产性能。产酸反应器的有机负载率 (OLR) 和 pH 值成功地用作调整参数以获得高发酵产量 (0.74 gCOD·gCOD ^–1) 和富含丁酸盐的发酵物，从而提高 PHA 生产步骤。作为将碳与氮供给解偶联的结果，选择了高度富含 PHA 储存微生物的生物质。生物量浓度达到了一个显着的值 (7.83 g·L ^–1 ) 作为对强加的高 OLR (8.7 gCOD·L ^–1 ·d ^–1 )的响应。在 PHA 积累试验中，在选择反应器中以最佳 OLR 选择的培养物实现了高储存产量（0.98 gCOD·gCOD ^–1），并且连续补料策略导致最大 PHA 含量为 80.5%（g 基） . 高全球生产率 (8.1 g-PHA·L ^–1 ·d ^–1 ) 和总工艺产量 (0.45 gCOD·gCOD ^–1) 是，据作者所知，使用中试规模的真实原料报告的 MMC 的最高值。这些结果证明了结合不同的有效策略以最大限度地提高工艺性能的重要性，这是从废物和 MMC 全面实施 PHA 生产的一个有希望的结果。

更新日期：2021-06-21

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