Loop diuretics are commonly used in clinical practice to manage high fluid loads and to control fluid balance. In this paper, a novel quantitative analysis method for multiple components with a single marker (QAMS) was developed for the simultaneous determination of 5 diuretic drugs furosemide, torasemide, azosemide, etacrynic acid, and bumetanide, by HPLC. Qualitative analysis was performed using relative retention time and ultraviolet (UV) spectral similarity as the double indicator. The QAMS method was conducted with etacrynic acid as an internal reference substance. The quantities of the other four diuretics were calculated by using the relative correction factors for etacrynic acid. The quantities of the 5 diuretic drugs were also determined by the external standard method (ESM). Chromatographic separation was achieved on a Shimadzu HC-C18 column (150 mm × 4.6 mm, 5 µm) using 50 mM potassium dihydrogen phosphate (pH adjusted to 4.0 with phosphoric acid) with acetonitrile (64:36, v/v) as the mobile phase at a flow rate of 1.0 mL/min and a column temperature of 30 ℃. Under these conditions, the 5 diuretic drugs were well separated, showing linear relationships within certain ranges. The quantitative results showed that there was no significant difference between the QAMS and ESM methods. Overall, the HPLC-QAMS analytical scheme established in this study is a simple, efficient, economical, and accurate method for the quantitative evaluation of 5 diuretic drugs.

中文翻译：

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使用单一标记物对多种成分进行定量分析，同时测定五种利尿药物

袢利尿剂在临床实践中常用于管理高液体负荷和控制液体平衡。本文建立了一种新的多组分单标记物定量分析方法 (QAMS)，用于通过 HPLC 同时测定 5 种利尿药物呋塞米、托拉塞米、阿佐塞米、依他尼酸和布美他尼。使用相对保留时间和紫外 (UV) 光谱相似性作为双重指标进行定性分析。QAMS 方法以依他宁酸为内标物质进行。其他四种利尿剂的量通过使用依他宁酸的相对校正因子计算。5种利尿药的用量也采用外标法（ESM）测定。在 Shimadzu HC-C18 色谱柱（150 mm × 4.6 mm，5 µm）上实现色谱分离，使用 50 mM 磷酸二氢钾（pH 用磷酸调节至 4.0），乙腈 (64:36, v/v) 作为流动相相，流速为 1.0 mL/min，柱温为 30 ℃。在此条件下，5种利尿药分离良好，在一定范围内呈线性关系。定量结果表明，QAMS 和 ESM 方法之间没有显着差异。总体而言，本研究建立的HPLC-QAMS分析方案是一种简单、高效、经济、准确的5种利尿药物定量评价方法。v/v) 作为流动相，流速为 1.0 mL/min，柱温为 30 ℃。在此条件下，5种利尿药分离良好，在一定范围内呈线性关系。定量结果表明，QAMS 和 ESM 方法之间没有显着差异。总体而言，本研究建立的HPLC-QAMS分析方案是一种简单、高效、经济、准确的5种利尿药物定量评价方法。v/v) 作为流动相，流速为 1.0 mL/min，柱温为 30 ℃。在此条件下，5种利尿药分离良好，在一定范围内呈线性关系。定量结果表明，QAMS 和 ESM 方法之间没有显着差异。总体而言，本研究建立的HPLC-QAMS分析方案是一种简单、高效、经济、准确的5种利尿药物定量评价方法。