Abstract—

This study examines the effect of glucocorticoid deflazacort on the functioning of rat liver mitochondria. Deflazacort at concentrations up to 100 μM has been shown to have no effect on respiration and oxidative phosphorylation of rat liver mitochondria, energized both in the presence of glutamate/malate (substrates of complex I of the respiratory chain) and succinate (substrate of complex II of the respiratory chain), and also does not affect activity of complexes of the respiratory chain of organelles. It was found that deflazacort does not affect the permeability of the inner membrane of liver mitochondria, but reduces the resistance of organelles to the induction of calcium-dependent MPT pore. In addition, we have found that this glucocorticoid is able to induce a decrease in the level of mitochondrial NAD(P)H, as well as inhibit the production of hydrogen peroxide by organelles. The paper discusses how the effects of deflazacort on mitochondrial function may be related to the therapeutic effects of this agent.

中文翻译：

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糖皮质激素地夫可特对大鼠肝线粒体呼吸和钙依赖性通透性的影响

摘要——

本研究考察了糖皮质激素地夫可特对大鼠肝线粒体功能的影响。已显示浓度高达 100 μM 的 Deflazacort 对大鼠肝线粒体的呼吸和氧化磷酸化没有影响，在谷氨酸/苹果酸（呼吸链复合物 I 的底物）和琥珀酸（复合物 II 的底物）存在下激发呼吸链），也不影响细胞器呼吸链复合物的活性。发现地夫可特不影响肝线粒体内膜的通透性，但降低细胞器对钙依赖性MPT孔诱导的抵抗力。此外，我们发现这种糖皮质激素能够诱导线粒体 NAD(P)H 水平的降低，以及抑制细胞器产生过氧化氢。该论文讨论了地夫可特对线粒体功能的影响如何与该药物的治疗效果相关。