In canine nutrition, the use of goat nutraceutical dairy products is an innovative proposal. Therefore, the objective of this study was to prepare fermented goat milk with probiotic potential in dogs in an in vitro model. A total of 40 lactic acid bacteria (LAB) species were grown, of which 30 were CAP isolates originally from goat milk and 10 were CAN isolates originally from fecal material of newborn dogs. The isolates were selected based on resistance to the simulated canine gastrointestinal condition and acidifying ability. After this preliminary screening, the analyses were performed regarding β-galactosidase and exopolysaccharide formation, diacetyl production, adhesion proteins Mub and mapa, hydrophobicity, DPPH assay, virulence and antibiotic resistance. With these evaluations, four LAB isolates were identified using sequencing of the 16S rRNA gene. These were identified as Enterococcus hirae and were used to produce fermented goat milk. For statistical analysis, the data were analyzed using the Scott-Knott test and also submitted to analysis of variance and the Tukey test (P < 0.05). In the evaluation of goat milk fermented with E. hirae and control, over the 36-day storage period there was a reduction in pH and an increase in acidity, and higher levels of LAB were observed in goat milk fermented with E. hirae. Therefore, both these E. hirae isolates and the fermented goat milk produced showed satisfactory results in vitro, demonstrating probiotic efficiency and food safety for dogs.

在犬类营养方面，使用山羊营养乳制品是一项创新提议。因此，本研究的目的是在体外为狗制备具有益生菌潜力的发酵山羊奶。模型。共培养了 40 种乳酸菌 (LAB)，其中 30 种是最初来自山羊奶的 CAP 分离物，10 种是最初来自新生犬粪便的 CAN 分离物。根据对模拟犬胃肠道状况的抵抗力和酸化能力选择分离株。在初步筛选后，对 β-半乳糖苷酶和胞外多糖的形成、双乙酰产生、粘附蛋白 Mub 和 mapa、疏水性、DPPH 测定、毒力和抗生素抗性进行了分析。通过这些评估，使用 16S rRNA 基因测序鉴定了四种 LAB 分离株。这些被鉴定为平肠球菌并被用于生产发酵山羊奶。对于统计分析，使用 Scott-Knott 检验分析数据，并提交方差分析和 Tukey 检验（P <  0.05）。在用E. hirae发酵的山羊奶和对照的评估中，在 36 天的储存期内，pH 值降低，酸度增加，并且在用E. hirae发酵的山羊奶中观察到更高水平的 LAB 。因此，这些E. hirae分离物和生产的发酵山羊奶在体外均显示出令人满意的结果，证明了狗的益生菌效率和食品安全性。