Foot rot/gummosis of citrus, caused by Phytophthora spp., is the most important disease of citrus, responsible for the yield losses of 10 to 30% in fruit production in major citrus growing countries. Considering the burden of disease, the present study was undertaken to evaluate different native potent strains of Trichoderma spp. against the foot rot/gummosis of citrus. Based on molecular characteristics, i.e., ITS ribosomal DNA sequence analysis, 3 isolates of Phytophthora nicotianae var. parasitica and 7 isolates of Trichoderma (6 isolates of T. asperellum and one of T. harzianum) were identified. Trichoderma isolates and Metalaxyl-M + Mancozeb were used to assess antagonistic activity against the foot rot/gummosis under field conditions. T. asperellum T20 mutant reduced gummosis lesion size (95.67%), improved feeder root density (0.0236 cm3), increased number (990.16), and yield of fruits (168.83 kg). The talc-based bioformulation of T20 mutant (T. asperellum) strain, used as a soil treatment, was able to manage effectively the gummosis/foot rot of citrus and also exhibited the highest yield.

中文翻译：

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木霉属强效天然菌株的生物制剂评估。对抗金诺柑的脚腐病/牙龈病

由 Phytophthora spp. 引起的柑橘类腐烂病/树胶病是柑橘类最重要的病害，导致主要柑橘类种植国的水果产量损失 10% 至 30%。考虑到疾病负担，本研究旨在评估木霉属的不同天然强效菌株。对抗柑橘的足部腐烂/胶质病。基于分子特征，即 ITS 核糖体 DNA 序列分析，烟草疫霉变种的 3 株分离物。鉴定了寄生虫和 7 株木霉（6 株 T. asperellum 和 1 株 T. harzianum）。木霉分离株和甲霜灵-M + 代森锰锌用于评估在田间条件下对抗足腐病/粘胶病的拮抗活性。T. asperellum T20 突变体减少了胶质病灶大小 (95.67%)，提高了饲养根密度 (0.0236 cm3)，增加数量（990.16）和果实产量（168.83 kg）。用作土壤处理的 T20 突变体 (T. asperellum) 菌株的基于滑石粉的生物制剂能够有效地控制柑橘的树胶病/腐烂病，并且还表现出最高的产量。