This study provides an innovative design to generate droplets in a single tube. This creative design utilizes a single tube to generate droplets for the emulsion Polymerase Chain Reaction (PCR). The multi-layered built-in microchannel structures were constructed in the disposable tube for minimum material used during the droplet PCR process. The PCR experiments show that the 3D tube provided a greater heating and cooling rate than the commercial PCR tube with the same system. In this research, droplets generation and PCR reaction results demonstrate the potentiality of digital PCR that can be executed in a single tubular structure.

中文翻译：

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具有液滴生成和温度控制的 3D 管状结构用于 DNA 扩增

这项研究提供了一种创新设计，可以在单管中产生液滴。这种创造性的设计利用单管为乳液聚合酶链反应 (PCR) 生成液滴。多层内置微通道结构在一次性管中构建，以在液滴 PCR 过程中使用最少的材料。PCR 实验表明，与具有相同系统的商业 PCR 管相比，3D 管提供了更大的加热和冷却速率。在这项研究中，液滴生成和 PCR 反应结果证明了可以在单个管状结构中执行的数字 PCR 的潜力。