The Aquaporins (AQPs) could prove to be striking targets of inflammation. The aim of this study was to study the involvement of AQPs and explore the anti-inflammatory activity of Garcinia extract in LPS induced acute systemic inflammation in Wistar rats. Adult male Wistar rats (n = 6) were pretreated with Garcinia orally twice for 7 days, followed by a single intraperitoneal dose (5.5 mg/kgbw) of LPS. Serum ALT, AST, ALP, Creatinine, Urea and BUN, nitric oxide, prostaglandin, cytokine and chemokine levels were measured. LC–MS analysis of Garcinia was performed to identify the phytoconstituents present. The iNOS and COX enzyme activity were determined in the target tissues. qPCR analysis of inos, cox-2 and aqps was performed. Relative protein expression of AQPs was studied by Western blot analysis. Molecular docking studies were performed to study the interaction of garcinol and hydroxycitric acid, the two important phytoconstituents of Garcinia with AQP. The qPCR analysis showed tissue-specific up-regulation of aqp1, aqp3, aqp4 and aqp8 in LPS induced rats. Garcinia extract treatment effectively lowered the mRNA expression of these AQPs. Garcinia extract significantly inhibited the LPS-induced NO, prostaglandin, cytokine and chemokine production in serum and also decreased tissue-specific transcript level of inos and cox-2, thus suggesting the anti-inflammatory role of Garcinia. Also, docking studies revealed interactions of garcinol and hydroxycitric acid with AQP1, 3, 4 and 8. Therefore, the present study suggests the possible involvement of AQP1, 3, 4 and 8 in inflammation and the efficacy of Garcinia extract as an anti-inflammatory agent. Therefore, AQPs can act as prognostic markers of inflammation and can be targeted with Garcinia extract.

水通道蛋白 (AQP) 可能被证明是炎症的显着目标。本研究的目的是研究 AQPs 的参与情况，并探讨藤黄提取物在 LPS 诱导的 Wistar 大鼠急性全身炎症中的抗炎活性。成年雄性 Wistar 大鼠 ( n  = 6)口服藤黄素两次，持续 7 天，然后单次腹腔注射 LPS (5.5 mg/kgbw)。测量血清ALT、AST、ALP、肌酐、尿素和BUN、一氧化氮、前列腺素、细胞因子和趋化因子水平。对藤黄进行LC-MS 分析以鉴定存在的植物成分。在靶组织中测定 iNOS 和 COX 酶活性。的qPCR分析的iNOS，COX-2和执行了aqps。通过蛋白质印迹分析研究 AQP 的相对蛋白质表达。进行分子对接研究以研究藤黄醇和羟基柠檬酸这两种重要的藤黄植物成分与 AQP的相互作用。qPCR分析显示LPS诱导的大鼠中aqp1、aqp3、aqp4和aqp8的组织特异性上调。藤黄提取物处理有效地降低了这些 AQP 的 mRNA 表达。藤黄提取物显着抑制 LPS 诱导的 NO、前列腺素、细胞因子和趋化因子在血清中的产生，并降低inos 和 cox-2 的组织特异性转录水平，从而提示藤黄的抗炎作用。此外，对接研究揭示了藤黄醇和羟基柠檬酸与 AQP1、3、4 和 8 的相互作用。因此，本研究表明 AQP1、3、4 和 8 可能参与炎症以及藤黄提取物作为抗炎药的功效代理人。因此，AQP 可以作为炎症的预后标志物，并且可以用藤黄提取物进行靶向。