Background: Indole-3-acetic acid (IAA) is an important growth hormone for plants obtained by biosynthesis from tryptophan.

Aim: In this paper was studied the competitive transport of two biologically active compounds, indole-3-acetic acid (IAA) and tryptophan (TRP), through a liquid membrane.

Methods: The separation of the two compounds was obtained using a hybrid liquid membrane system having trioctylphosphine oxide (TOPO) as a carrier.

Results: The most important operational parameters of the system, and pH influence on the efficiency of the transport process, in correlation with the speciation diagrams of the two compounds: TRP and IAA, were studied. The evaluation of the transport process was performed by calculating the composition of the phases at the end of the transport process and the organic substrate flow at the membranes exit.

Conclusion: Due to the transport efficiency of over 90% in the case of IAA and the high selectivity at the transport between IAA and TRP, the procedure can be applied for the preparation of a sample containing these analytes.

背景：吲哚-3-乙酸（IAA）是一种重要的植物生长激素，通过色氨酸生物合成获得。

目的：本文研究了两种生物活性化合物吲哚-3-乙酸（IAA）和色氨酸（TRP）通过液膜的竞争转运。

方法：使用三辛基氧化膦 (TOPO) 作为载体的混合液膜系统实现了两种化合物的分离。

结果：研究了系统最重要的操作参数和 pH 值对传输过程效率的影响，并与两种化合物：TRP 和 IAA 的形态图相关联。传输过程的评估是通过计算传输过程结束时的相组成和膜出口处的有机底物流量来进行的。

结论：由于 IAA 的传输效率超过 90%，并且 IAA 和 TRP 之间的传输具有高选择性，因此该程序可用于制备含有这些分析物的样品。