A novel redox-active Schiff base polymer (SBP_PPDA-PDA) was prepared by condensation of p-phenylenediamine with 1,10-phenanthroline-2,9-dicarbaldehyde. The structure of SBP_PPDA-PDA was investigated by scanning electron microscopy, transmission electron microscopy, atomic force microscopy, Fourier transform infrared spectroscopy, N₂ adsorption/desorption isotherm, and powder X-ray diffraction and electrochemical techniques. The results showed that SBP_PPDA-PDA had two-dimensional lamellar structure. The electrochemical properties of SBP_PPDA-PDA were characterized by cyclic voltammetry, showing several pairs of redox peaks and good catalytic ability toward oxygen reduction. On the basis of the redox-active activity of SBP_PPDA-PDA, an electrochemical pH sensor was proposed based on SBP_PPDA-PDA with a linear range from 2 to 9 for pH detection. With nitrogen-containing skeleton and porous structure, SBP_PPDA-PDA could be used as support to load glucose oxidase (GOD). Thus, a novel ratiometric electrochemical glucose sensor was further constructed by immobilizing GOD on SBP_PPDA-PDA modified electrode. The results revealed that the reduction peak of O₂ decreased with the gradual addition of glucose, while the response peak of SBP_PPDA-PDA at about 0.15 V almost remained. The sensor based on GOD/SBP_PPDA-PDA/SPE showed a rapid response, a linear range from 22.5 μmol/L–5 mmol/L and a lower detection limit of 7.56 μmol/L for glucose.

通过对苯二胺与 1,10-菲咯啉-2,9-二甲醛缩合制备新型氧化还原活性席夫碱聚合物 (SBP _PPDA-PDA ) 。通过扫描电子显微镜、透射电子显微镜、原子力显微镜、傅里叶变换红外光谱、N ₂吸附/解吸等温线、粉末X射线衍射和电化学技术研究了SBP _PPDA-PDA的结构。结果表明SBP _PPDA-PDA具有二维层状结构。SBP _PPDA-PDA的电化学性能用循环伏安法表征，显示出几对氧化还原峰和良好的氧还原催化能力。基于SBP _PPDA-PDA的氧化还原活性，提出了一种基于SBP _PPDA-PDA的电化学pH传感器，线性范围为2~9，用于pH检测。SBP _PPDA-PDA具有含氮骨架和多孔结构，可作为负载葡萄糖氧化酶（GOD）的载体。因此，通过将 GOD 固定在 SBP _PPDA-PDA修饰电极上，进一步构建了一种新型比率电化学葡萄糖传感器。结果表明，O ₂的还原峰随着葡萄糖的逐渐加入而降低，而SBP _PPDA-PDA的响应峰几乎保持在 0.15 V 左右。基于 GOD/SBP _PPDA-PDA /SPE的传感器显示出快速响应，线性范围为 22.5 μmol/L–5 mmol/L，葡萄糖检测下限为 7.56 μmol/L。