当前位置：
X-MOL 学术
›
Ann. Appl. Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Translationally controlled tumour protein: A protein necessary for potyvirus intracellular multiplication that supports plant infection by unrelated viruses
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-06-09 , DOI: 10.1111/aab.12708 Fernanda Prieto Bruckner 1, 2, 3 , Patrícia Oliveira Andrade 1 , Renan Souza Cascardo 1 , Jean‐François Laliberté 4 , Poliane Alfenas‐Zerbini 1
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-06-09 , DOI: 10.1111/aab.12708 Fernanda Prieto Bruckner 1, 2, 3 , Patrícia Oliveira Andrade 1 , Renan Souza Cascardo 1 , Jean‐François Laliberté 4 , Poliane Alfenas‐Zerbini 1
Affiliation
|
The multifunctional protein translationally controlled tumour protein (TCTP) was previously identified as necessary for infection by the potyvirus pepper yellow mosaic virus. Using turnip mosaic virus (TuMV) as a model to study potyvirus biology, we confirmed that TCTP has a positive effect on virus infection. Living cell confocal microscopy demonstrated that TCTP colocalises with 6K2-tagged replication vesicles and with a perinuclear globular structure typically observed during potyvirus infection. Also, TCTP silenced protoplasts showed reduced virus accumulation, quantified by qRT-PCR, which suggests an effect on virus replication, translation or other intracellular process. Finally, TCTP silencing in plants reduced the accumulation of two species belonging to Orthotospovirus and a Begomovirus genus, which are not closely related to potyviruses. The results suggest that TCTP is a general susceptibility factor to several unrelated viruses.
中文翻译：
翻译控制的肿瘤蛋白：一种支持植物感染无关病毒的potyvirus细胞内增殖所必需的蛋白质
多功能蛋白翻译控制的肿瘤蛋白 (TCTP) 以前被确定为感染辣椒黄花叶病毒所必需的。以芜菁花叶病毒（TuMV）为模型研究马铃薯病毒生物学，证实TCTP对病毒感染具有积极作用。活细胞共聚焦显微镜表明 TCTP 与 6K2 标记的复制囊泡共定位，并具有在马病毒感染期间通常观察到的核周球状结构。此外，TCTP沉默的原生质体显示病毒积累减少，通过 qRT-PCR 量化，这表明对病毒复制、翻译或其他细胞内过程的影响。最后，植物中的TCTP沉默减少了属于Orthotospovirus和Begomovirus属，它们与potyviruses 没有密切关系。结果表明，TCTP 是对几种不相关病毒的普遍易感性因素。
更新日期：2021-06-09
中文翻译：
翻译控制的肿瘤蛋白：一种支持植物感染无关病毒的potyvirus细胞内增殖所必需的蛋白质
多功能蛋白翻译控制的肿瘤蛋白 (TCTP) 以前被确定为感染辣椒黄花叶病毒所必需的。以芜菁花叶病毒（TuMV）为模型研究马铃薯病毒生物学，证实TCTP对病毒感染具有积极作用。活细胞共聚焦显微镜表明 TCTP 与 6K2 标记的复制囊泡共定位，并具有在马病毒感染期间通常观察到的核周球状结构。此外，TCTP沉默的原生质体显示病毒积累减少，通过 qRT-PCR 量化，这表明对病毒复制、翻译或其他细胞内过程的影响。最后，植物中的TCTP沉默减少了属于Orthotospovirus和Begomovirus属，它们与potyviruses 没有密切关系。结果表明，TCTP 是对几种不相关病毒的普遍易感性因素。
京公网安备 11010802027423号