To investigate biomarkers representing the physiological and biochemical responses of the brackish-water clam Corbicula japonica, we conducted a full factorial-design experiment to test different water-temperature levels (20 °C and 25 °C), salinity levels (5 and 20 psu), and food-availability levels (0.5 and 2.0 mg suspended solids (SS)·ind⁻¹·d⁻¹). Increase in water temperature significantly decreased superoxide dismutase (SOD) and catalase (CAT) activities and oxyradical-absorbance capacity (ORAC), leading to lipid peroxidation (i.e., oxidative damage). Salinity activated or inhibited these biochemical markers. Food availability supported a detoxification mechanism against oxidative stress. Principal-components and cluster analyses revealed that a total of eight experimental conditions fell into three groups related to water temperature and/or salinity. The shift from Group I (20 °C water temperature) to Group II (25 °C water temperature and 5-psu salinity) demonstrated that the condition index, SOD, CAT, and ORAC had significantly decreased. With the further shift to Group III (25 °C water temperature and 20-psu salinity), we found a prominent increase in ORAC, which led to oxidative damage but no mortality. We conclude that future habitat changes driven by global warming should be closely watched, particularly given that local anthropogenic disturbances further add to natural ones.

为了研究代表咸水蛤Corbicula japonica生理和生化反应的生物标志物，我们进行了一项全因子设计实验，以测试不同的水温水平（20°C 和 25°C）、盐度水平（5 和 20 psu） ) 和食物供应水平（0.5 和 2.0 毫克悬浮固体 (SS)·ind ^-1 ·d ^-1）。水温升高会显着降低超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 的活性以及氧自由基吸收能力 (ORAC)，从而导致脂质过氧化（即氧化损伤）。盐度激活或抑制这些生化标志物。食物供应支持抗氧化应激的解毒机制。主成分和聚类分析表明，共有八个实验条件分为与水温和/或盐度相关的三组。从组 I（20 °C 水温）到组 II（25 °C 水温和 5 psu 盐度）的转变表明条件指数、SOD、CAT 和 ORAC 显着降低。随着进一步转向第三组（25 °C 水温和 20 psu 盐度），我们发现 ORAC 显着增加，这导致氧化损伤，但没有死亡。我们得出的结论是，应密切关注由全球变暖驱动的未来栖息地变化，特别是考虑到当地人为干扰进一步增加了自然干扰。