We proposed selective manipulation techniques for retrieving and retaining target cells arrayed in microwells based on dielectrophoresis (DEP). The upper substrate with microband electrodes was mounted on the lower substrate with microwells based on the same design of microband electrodes by 90 degree relative to the lower substrate. A repulsive force of negative dielectrophoresis (n-DEP) was employed to retrieve the target cells from the microwell array selectively. Furthermore, the target cells were retained in the microwells after other cells were removed by n-DEP. Thus, the system described in this study could make it possible to retrieve and recover single target cells from a microwell array after determining the function of cells trapped in each microwell.

我们提出了基于介电泳 (DEP) 的选择性操作技术，用于检索和保留排列在微孔中的靶细胞。具有微带电极的上基板安装在具有基于相同微带电极设计的微孔的下基板上，相对于下基板成 90 度。负介电泳排斥力 (n-DEP) 被用来选择性地从微孔阵列中检索目标细胞。此外，在其他细胞被 n-DEP 去除后，靶细胞仍保留在微孔中。因此，本研究中描述的系统可以在确定每个微孔中捕获的细胞的功能后，从微孔阵列中检索和恢复单个目标细胞。