The objective of this study was to evaluate the antimicrobial effect in vitro of an acetonic-methanol extract (1:1 v/v) from Hibiscus sabdariffa L. encapsulated calyxes using a mixture of modified corn starch (MCS) and pectin as a wall material. Previously, the MCS was hydrolyzed-succinated and processed into a single-screw extruder. For H. sabdariffa extract encapsulation, optimal drying conditions were established using encapsulation efficiency (EE) and antimicrobial activity against Salmonella typhimurium and Staphyloccocus aureus as response variables.

Optimum drying conditions were total soluble solids concentration of 10 % (MCS 9% / Pectin 1%) at drying temperature of 120 °C with a maximum EE of 98.5 %.

H. sabdariffa extract obtained from solvent blend produced macromolecules extraction that favor the synergism for antimicrobial activity against S. typhimurium and S. aureus with inhibition halos of 19.9 and 20.9 mm, only in extract. While the microencapsuled extract exhibited inhibition halos of 16.8 and 19.9 mm, respectively. The microencapsulates with higher pectin content significantly increased its antimicrobial activity (p ≤ 0.05) as compare to those encapsulates with lower pectin concentrations. This process is due to synergistic reorganizations of the molecular formations of both polymers and the extract leading to a diffusion of antimicrobial compounds. The microcapsules of the acetonic-metabolic extract of Hibiscus sabdariffa obtained in this work are a naturally sourced alternative for the conservation of food products.

本研究的目的是评估使用改性玉米淀粉 (MCS) 和果胶的混合物作为壁材料的木槿包囊花萼丙酮-甲醇提取物 (1:1 v/v) 的体外抗菌作用. 以前，MCS 被水解琥珀酸并加工成单螺杆挤出机。对于H. sabdariffa提取物封装，使用封装效率 (EE) 和对鼠伤寒沙门氏菌和金黄色葡萄球菌的抗菌活性作为响应变量来建立最佳干燥条件。

最佳干燥条件是总可溶性固体浓度为 10%（MCS 9%/果胶 1%），干燥温度为 120°C，最大 EE 为 98.5%。

从混合溶剂中获得的虎耳草提取物产生的大分子提取物有利于对鼠伤寒沙门氏菌和金黄色葡萄球菌的抗菌活性产生协同作用，抑制光晕为 19.9 和 20.9 毫米，仅在提取物中。而微胶囊提取物的抑制光晕分别为 16.8 和 19.9 毫米。与果胶浓度较低的微胶囊相比，果胶含量较高的微胶囊显着提高了其抗菌活性 ( p ≤ 0.05)。该过程是由于聚合物和提取物的分子结构协同重组导致抗菌化合物扩散。丙酮代谢提取物的微胶囊在这项工作中获得的Hibiscus sabdariffa是一种天然来源的食品保护替代品。