Abstract

This work describes the fabrication of quantum dot (QD)/silica (SiO₂) core–shell particles immobilized with Au nanoparticles and the evaluation of their abilities in fluorescence and x-ray computed tomography (CT) imaging. The QD nanoparticles were coated with silica shells by a sol-gel method using tetraethyl orthosilicate (QD/SiO₂). Reduction of Au³⁺ in a citrate aqueous solution gave an Au nanoparticle colloidal solution. (3-Mercaptopropyl)trimethoxysilane thiolated the QD/SiO₂ particle surface (QD/SiO₂/SH). Immobilization of the Au nanoparticles on the QD/SiO₂/SH particle surface was performed with the addition of the Au nanoparticle colloidal solution to the QD/SiO₂/SH nanoparticle colloidal solution (QD/SiO₂/SH/Au). Surface-PEGylated QD/SiO₂/SH/Au particles were fabricated using thiol-terminated polyethylene glycol (PEG) (QD/SiO₂/SH/Au/PEG). The QD/SiO₂/SH/Au/PEG nanoparticle colloidal solution was imaged by fluorescence and x-ray CT imaging techniques. The CT value per unit concentration of Au (M) for the QD/SiO₂/SH/Au/PEG nanoparticle colloidal solution was 1.5 × 10⁴ HU/M. The QD/SiO₂/SH/Au/PEG nanoparticle colloidal solution was injected into the tail vein of a mouse and circulated in the blood, although some QD/SiO₂/SH/Au/PEG nanoparticles were trapped and remained in the liver and spleen.

摘要

这项工作描述了用金纳米粒子固定的量子点 (QD)/二氧化硅 (SiO ₂ ) 核壳粒子的制造，以及它们在荧光和 X 射线计算机断层扫描 (CT) 成像中的能力评估。使用原硅酸四乙酯 (QD/SiO ₂ ) 通过溶胶-凝胶法将 QD 纳米颗粒涂有二氧化硅壳。将柠檬酸盐水溶液中的Au ³⁺还原得到Au纳米粒子胶体溶液。(3-巯基丙基)三甲氧基硅烷硫醇化QD/SiO ₂颗粒表面(QD/SiO ₂ /SH)。通过将金纳米颗粒胶体溶液添加到QD/SiO ₂ /SH 颗粒表面上进行Au 纳米颗粒的固定。₂ /SH纳米粒子胶体溶液（QD/SiO ₂ /SH/Au）。使用硫醇封端的聚乙二醇 (PEG) (QD/SiO ₂ /SH/Au/PEG)制造表面聚乙二醇化的 QD/SiO _{2 /SH/Au 颗粒。}通过荧光和X射线CT成像技术对QD/SiO _{2 /SH/Au/PEG纳米颗粒胶体溶液进行成像。QD/SiO 2} /SH/Au/PEG纳米粒子胶体溶液每单位浓度Au(M)的CT值为1.5×10 ⁴ HU/M。将 QD/SiO ₂ /SH/Au/PEG 纳米颗粒胶体溶液注入小鼠尾静脉并在血液中循环，尽管一些 QD/SiO ₂/SH/Au/PEG 纳米颗粒被捕获并保留在肝脏和脾脏中。