Plant Foods for Human Nutrition ( IF 3.1 ) Pub Date : 2021-06-09 , DOI: 10.1007/s11130-021-00902-x Branislava Teofilović 1 , Nevena Grujić-Letić 2 , Emilia Gligorić 1 , Aleksandar Rašković 1 , Ružica Igić 2 , Gyöngyi Vastag 2 , Slobodan Gadžurić 2
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Abstract
The possibility to prevent nutrition-related diseases that include scavenge of free radicals and to block chain reactions is very important and significant for human well-being. The aim of this study was to analyse different basil extracts, determine the relationship between total phenolic/flavonoid content and antioxidant activity in order to optimize its application in industry. The extraction involved different solvents (ethanol, methanol and water), extraction time (10 and 30 min and 24, 48 and 72 h), plant fragmentation level (0.3 and 2 mm) and the presence or absence of light. Antioxidant activity was investigated by applying spectrophotometric method and measuring the total phenolic and flavonoid content and DPPH radical scavenging activity. The content of total phenolics varied from 5.2 to 185.6 mg of gallic acid equivalents per gram of a dry extract and flavonoids ranged from 0.2 to 35.0 mg of quercetin per gram of a dry extract. All extracts presented a scavenging capacity and IC50 values of DPPH radical inhibition ranged from 0.04 to 12.99 μg/ml. The evaluation of experimental data for eighty basil extracts was performed by chemometric analysis showing good correlation between yield and total phenolic compounds, as well as flavonoid content and inhibition of the DPPH radical.
Graphical abstract
中文翻译：
甜罗勒提取物 (Ocimum basilicum L.) 提取程序和清除能力的实验和计算评估
摘要
预防包括清除自由基和阻断链反应在内的营养相关疾病的可能性对人类福祉非常重要。本研究的目的是分析不同的罗勒提取物，确定总酚/黄酮含量与抗氧化活性之间的关系，以优化其在工业中的应用。提取涉及不同的溶剂（乙醇、甲醇和水）、提取时间（10 和 30 分钟以及 24、48 和 72 小时）、植物破碎水平（0.3 和 2 毫米）以及光的存在与否。通过应用分光光度法并测量总酚和黄酮含量以及DPPH自由基清除活性来研究抗氧化活性。总酚含量从 5.2 到 185 不等。每克干提取物相当于 6 毫克没食子酸，而每克干提取物含有 0.2 至 35.0 毫克槲皮素。所有提取物都具有清除能力和 ICDPPH 自由基抑制的50值范围为 0.04 至 12.99 μg/ml。通过化学计量学分析对八十种罗勒提取物的实验数据进行评估，结果表明产量与总酚类化合物、黄酮类化合物含量和 DPPH 自由基抑制之间具有良好的相关性。
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