Analysis of Antimicrobial and Antibiofilm Activity of Human Milk Lactoferrin Compared to Bovine Lactoferrin against Multidrug Resistant and Susceptible Acinetobacter baumannii Clinical Isolates,ACS Infectious Diseases

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Analysis of Antimicrobial and Antibiofilm Activity of Human Milk Lactoferrin Compared to Bovine Lactoferrin against Multidrug Resistant and Susceptible Acinetobacter baumannii Clinical Isolates
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2021-06-09 , DOI: 10.1021/acsinfecdis.1c00087
Tyra M Avery ₁ , RaNashia L Boone ₁ , Jacky Lu ₂ , Sabrina K Spicer ₃ , Miriam A Guevara ₂ , Rebecca E Moore ₃ , Schuyler A Chambers ₃ , Shannon D Manning ₄ , Leon Dent _{5,

6} , Dana Marshall ₅ , Steven M Damo _{1,

7,

8} , Steven D Townsend ₃ , Jennifer A Gaddy _{2,

9,

10}

Affiliation

Department of Life and Physical Sciences, Fisk University, Talley-Brady Hall, 1000 17th Ave. N, Nashville, Tennessee 37208, United States.
Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37212, United States.
Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, United States.
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824, United States.
Department of Pathology, Anatomy, and Cell Biology, Meharry Medical College, Nashville, Tennessee 37208, United States.
Trauma Services, Phoebe Putney Memorial Hospital, Albany, Georgia 31701, United States.
Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37205, United States.
Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37205, United States.
Department of Medicine, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, Tennessee 37232, United States.
Department of Veterans Affairs, Tennessee Valley Healthcare Systems, Nashville, Tennessee 37212, United States.

Acinetobacter baumannii is an opportunistic bacterial pathogen that causes severe infections in immunocompromised patients. The emergence of multi- and pan-drug resistant strains of A. baumannii from clinical sources has confounded treatment and enhanced morbidity and mortality associated with these infections. One way that A. baumannii circumnavigates environmental and antimicrobial challenge is by forming tertiary architectural structures of cells known as biofilms. Biofilm-inhibiting molecules could be deployed as a potential chemotherapeutic strategy to inhibit or disrupt A. baumannii biofilms and mitigate adverse outcomes due to infection. Lactoferrin is an innate immune glycoprotein produced in high concentrations in both human and bovine milk which has previously been shown to have antibacterial and antibiofilm activities. We sought to test lactoferrin against a bank of clinical isolates of A. baumannii to determine changes in bacterial growth or biofilm formation. Our results indicate that human lactoferrin has slightly more potent antibacterial activities than bovine lactoferrin against certain strains of A. baumannii and that these effects are associated with anatomical site of isolation. Additionally, we have shown that both bovine and human lactoferrin can inhibit A. baumannii biofilm formation and that these effects are associated with anatomical site of isolation and whether the strain forms robust or weak biofilms.

中文翻译：

人乳乳铁蛋白与牛乳铁蛋白相比对多药耐药和易感鲍曼不动杆菌临床分离株的抗菌和抗菌活性分析

鲍曼不动杆菌是一种机会性细菌病原体，可在免疫功能低下的患者中引起严重感染。来自临床来源的多重和泛耐药鲍曼不动杆菌菌株的出现混淆了治疗，并增加了与这些感染相关的发病率和死亡率。A. baumannii绕过环境和抗菌挑战的一种方法是形成称为生物膜的细胞的三级建筑结构。生物膜抑制分子可用作抑制或破坏鲍曼不动杆菌的潜在化疗策略生物膜并减轻感染引起的不良后果。乳铁蛋白是一种先天免疫糖蛋白，在人和牛奶中均以高浓度产生，此前已证明具有抗菌和抗生物膜活性。我们试图针对一组鲍曼不动杆菌临床分离株测试乳铁蛋白，以确定细菌生长或生物膜形成的变化。我们的研究结果表明，人乳铁蛋白对某些鲍曼不动杆菌菌株的抗菌活性略强于牛乳铁蛋白，并且这些作用与解剖部位的隔离有关。此外，我们已经证明牛和人乳铁蛋白都可以抑制鲍曼不动杆菌生物膜的形成，这些影响与解剖部位的隔离以及菌株是否形成坚固或薄弱的生物膜有关。

更新日期：2021-08-13

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