ABSTRACT

Grifola frondosa (G. frondosa) is an edible mushroom consumed as a health food in Asia that has recently been reported to have anti-allergic effects. In this study, we investigated the effects of G. frondosa on pollen allergy-induced ocular itching in mice. G. frondosa was boiled and freeze-dried (heat-treated G. frondosa). Oral administration of heat-treated G. frondosa (0.1 and 1 g/kg) suppressed pollen allergy-induced ocular itching and plasma extravasation but not histamine-induced plasma extravasation. Oral administration of ergosterol, an active ingredient in G. frondosa (5 and 50 mg/kg), or zileuton (100 mg/kg), which is an inhibitor of 5-lipoxygenase (the main enzyme required for leukotriene B₄ production), inhibited pollen allergy-induced ocular itching. In addition, ergosterol inhibited antigen-induced leukotriene B₄ production in mast cells. These findings suggest that G. frondosa and its active ingredient ergosterol prevent pollen allergy-induced itching through the inhibition of leukotriene B₄ production in mast cells.

摘要

灰树花( G. frondosa ) 是一种在亚洲作为保健食品食用的食用菌，最近有报道称其具有抗过敏作用。在这项研究中，我们研究了绿树花对花粉过敏引起的小鼠眼痒的影响。将 G. frondosa煮沸并冷冻干燥（热处理G. frondosa）。口服经热处理的绿树花（0.1 和 1 g/kg）可抑制花粉过敏引起的眼痒和血浆外渗，但不能抑制组胺引起的血浆外渗。G. frondosa 中的一种活性成分麦角甾醇的口服给药（5 和 50 毫克/千克）或齐留通（100 毫克/千克）是 5-脂氧合酶（产生白三烯 B ₄所需的主要酶）的抑制剂，可抑制花粉过敏引起的眼部瘙痒。此外，麦角甾醇抑制肥大细胞中抗原诱导的白三烯 B ₄产生。这些发现表明G. frondosa及其活性成分麦角甾醇通过抑制肥大细胞中白三烯 B _{4 的}产生来预防花粉过敏引起的瘙痒。