Landmark and sustainable eco-friendly dye treatment processes are highly desirous to ameliorate their effect on the environment. The present study investigated the azo dye degradation efficiency of adapted Enterobacter hormaechei SKB 16 from textile effluent polluted soil in optimized culture conditions. The adapted bacteria strain was identified by standard microbiological and molecular techniques. E. hormaechei was tested individually for the decolourizing of Reactive Yellow 145 (RY 145) and Reactive Red F3B (RR 180) dyes under optimized conditions of pH, temperature and dye concentration on decolourization were studied. The adapted bacteria strain exhibited maximum decolourization (98 %) of Reactive yellow 145 and Reactive red 180 in 100 ppm concentration at pH 7, temperature 37 °C after 98 h of incubation. The enzyme analyses revealed that azo reductase and laccase played major roles in the cleavage of the azo bond and desulfonation respectively of both dyes during degradation. The metabolites were further characterized by Fourier Transform Infrared Spectroscopy (FT-IR), High-Performance Liquid Chromatography (HPLC), and Gas Chromatography-Mass Spectrometry (GCMS). Thereafter, degradation was deduced based on changes of the functional group, variation in retention times and mass/charge ratio and molecular weight. This study elucidated the promising potentials of adapted SKB 16 strain in the eco-friendly removal of textile azo dyes. In addition, repeatability and sustainability are enhanced due to effective management of time which would have been spent on rigorous and extensive screening process.

具有里程碑意义且可持续的环保染料处理工艺非常需要改善它们对环境的影响。本研究调查了在优化培养条件下，来自纺织废水污染土壤的适应性肠杆菌 hormaechei SKB 16的偶氮染料降解效率。通过标准微生物学和分子技术鉴定适应的细菌菌株。E. hormaechei分别测试了活性黄 145 (RY 145) 和活性红 F3B (RR 180) 染料在优化的 pH 值、温度和染料浓度对脱色的影响。适应的细菌菌株在 100 ppm 浓度、pH 7、温度 37 °C 下，在孵育 98 小时后表现出最大程度的脱色 (98%) 活性黄 145 和活性红 180。酶分析表明，偶氮还原酶和漆酶分别在降解过程中两种染料的偶氮键断裂和脱磺化中起主要作用。通过傅里叶变换红外光谱 (FT-IR)、高效液相色谱 (HPLC) 和气相色谱-质谱 (GC) 进一步表征代谢物多发性硬化症）。此后，根据官能团的变化、保留时间和质荷比和分子量的变化推断降解。这项研究阐明了适应的 SKB 16 菌株在环保去除纺织偶氮染料方面的潜力。此外，由于有效管理本应用于严格和广泛筛选过程的时间，可重复性和可持续性得到增强。