Viral respiratory rapid multiplex PCR assays FilmArray® (FA) and ePlex® (eP) provide qualitative results which may not reflect clinical relevance. In a pilot study, we report retrospectively whether the semi-quantitative PCR assay R-GENE® would have facilitated clinical interpretation. Forty-four patients were hospitalized for various respiratory manifestations; all of them have benefited from a respiratory sample during acute symptoms. Among the 44 patients, FA detected 23 positive samples including 31 viruses, 26 of them gave high or moderate R-GENE® scores (cycle threshold < 35), and all but one were consistent with clinical history. Semi-quantitative scores would have allowed for critical interpretation of the results; those are a key additional element for an optimal exploitation of the rapid multiplex PCR assays power.

病毒呼吸快速多重 PCR 检测 FilmArray® (FA) 和 ePlex® (eP) 提供的定性结果可能无法反映临床相关性。在一项试点研究中，我们回顾性报告了半定量 PCR 检测 R-GENE® 是否有助于临床解释。44 名患者因各种呼吸道表现住院；他们所有人都从急性症状期间的呼吸道样本中受益。在 44 名患者中，FA 检测到 23 份阳性样本，包括 31 种病毒，其中 26 名给出了高或中 R-GENE® 评分（周期阈值 < 35），除 1 名外，其余均与临床病史一致。半定量分数将允许对结果进行批判性解释；这些是优化利用快速多重 PCR 分析能力的关键附加要素。