Differential phase contrast (DPC) microscopy provides isotropic phase images by applying asymmetric illumination patterns on the sample. The movement of specimens during series image acquisition may lead to motion blur artifacts, which are difficult to prevent. Here, we propose a new method based on pupil engineering and color multiplexing to obtain an isotropic phase transfer function and to reduce the required frames simultaneously. Radially asymmetric color pupils are implemented in a DPC microscope using a programmable thin-film transistor as a digital pupil, which gives flexibility and dynamic control for projecting illumination patterns on samples. With our approach, an isotropic quantitative phase map can be obtained using only pairwise color images for phase reconstruction. A radially asymmetric color pupil is synthesized by encoding the red, green, and blue colors. To recover accurate phase values, a color-leakage correction algorithm is applied to calibrate each color channel. Compared to a half-circle illumination pupil, our method can significantly enhance the image acquisition speed. The phase recovery accuracy is more than 97%. To show the imaging performance of our proposed method, quantitative phase imaging of living 3T3 mouse fibroblast cells is performed. Our quantitative phase measurement method may find important applications in biomedical research.

微分相差 (DPC) 显微镜通过在样品上应用非对称照明图案来提供各向同性相位图像。系列图像采集过程中标本的移动可能会导致运动模糊伪影，这是难以防止的。在这里，我们提出了一种基于瞳孔工程和颜色复用的新方法，以获得各向同性的相位传递函数并同时减少所需的帧。使用可编程薄膜晶体管作为数字瞳孔，在 DPC 显微镜中实现了径向非对称色瞳，这为在样品上投射照明图案提供了灵活性和动态控制。通过我们的方法，可以仅使用成对的彩色图像进行相位重建来获得各向同性的定量相位图。通过对红色、绿色和蓝色进行编码来合成径向非对称色瞳。为了恢复准确的相位值，应用颜色泄漏校正算法来校准每个颜色通道。与半圆照明瞳孔相比，我们的方法可以显着提高图像采集速度。相位恢复精度超过97%。为了显示我们提出的方法的成像性能，对活的 3T3 小鼠成纤维细胞进行了定量相位成像。我们的定量相位测量方法可能会在生物医学研究中找到重要的应用。相位恢复精度超过97%。为了显示我们提出的方法的成像性能，对活的 3T3 小鼠成纤维细胞进行了定量相位成像。我们的定量相位测量方法可能会在生物医学研究中找到重要的应用。相位恢复精度超过97%。为了显示我们提出的方法的成像性能，对活的 3T3 小鼠成纤维细胞进行了定量相位成像。我们的定量相位测量方法可能会在生物医学研究中找到重要的应用。