Graphene-based materials are capable of enhancing the refractometric response of prism- and optical fiber-based surface plasmon resonance (SPR) sensors; however, complicated multistep and time-consuming attaching processes could limit their practical applications. Herein, for the first time, we demonstrate the immobilization of graphene oxide (GO) submicrometric sheets onto the surface of a gold-coated single-mode fiber using a coating of fungal self-assembling proteins, the hydrophobins (HFBs), as an adhesive nanolayer. Hetero-core fiber tip SPR structures used in this study, consisting of a mirrored multimode–single-mode fiber structure coated with different thin layers (a chromium layer of 3 nm and a gold layer of 30 nm on top) exhibited a refractive index sensitivity (S_RI) of 1842 nm RIU⁻¹ (RIU: refractive index unit) at a refractive index (RI) of 1.36. Self-assembly of GO over the SPR fiber tip via HFB, offered an enhancement of up to 20% in the S_RI. Moreover, this HFB-GO coating prevented degradation of the Al thin film mirror caused by corrosive salt-water solutions. The process is very simple, harmless, rapid (around 15 min) and scalable, as it is mostly based on one plasma treatment, which can be performed in large chambers and two dip coating steps, in liquid baths. All these features make the use of self-assembled bio/non-bio hybrid coating a green industrial method to improve the performance of SPR fiber biosensors, if compared with traditional chemical methods. Materials applied in this technology, fungal proteins and derivatives of graphite, are sustainable and largely available.

基于石墨烯的材料能够增强基于棱镜和光纤的表面等离子体共振 (SPR) 传感器的折光响应；然而，复杂的多步骤和耗时的连接过程可能会限制它们的实际应用。在此，我们首次展示了使用真菌自组装蛋白、疏水蛋白 (HFB) 作为粘合剂将氧化石墨烯 (GO) 亚微米片固定在镀金单模光纤的表面上纳米层。本研究中使用的异芯光纤尖端 SPR 结构由涂覆有不同薄层（3 nm 铬层和 30 nm 金层）的镜像多模-单模光纤结构组成，表现出折射率敏感性(S _RI ) 1842 nm RIU ^-1(RIU: 折射率单位) 的折射率 (RI) 为 1.36。通过 HFB 在 SPR 光纤尖端上自组装 GO，在 S _{RI 中}提供了高达 20% 的增强。此外，这种 HFB-GO 涂层防止了由腐蚀性盐水溶液引起的铝薄膜镜的退化。该过程非常简单、无害、快速（约 15 分钟）且可扩展，因为它主要基于一种等离子体处理，可以在大腔室和两个浸涂步骤中，在液体浴中进行。与传统化学方法相比，所有这些特点使自组装生物/非生物混合涂层的使用成为一种绿色工业方法，可提高 SPR 纤维生物传感器的性能。应用于这项技术的材料，真菌蛋白和石墨衍生物，是可持续的，并且大量可用。