In the present study, we describe a new silicon phthalocyanine conjugated to bovine serum albumin (PcSiN3M-BSA) and its photodynamic activity in murine macrophages cells (J774.A1). The nonconjugated precursor, bis(trimethylaminoethanoxy)–phthalocyaninato silicon (IV) (PcSiN3M), was also studied. Compounds PcSiN3M and PcSiN3M-BSA showed no cytotoxicity in the dark, but exhibited high photodynamic activities following exposure to 5 M photosensitizers and 45 J cm⁻² irradiation. These conditions were sufficient to decrease the cell viability to 40% and 5% in cells treated with PcSiN3M and PcSiN3M-BSA, respectively. These results demonstrated an increase of 87% in the photodynamic activity of PcSiN3M when conjugated with BSA. The results shown in this work suggest that PcSiN3M-BSA had higher uptake by J774.A1 cells, which contributed to its higher photoactivity compared with the unconjugated form, PcSiN3N.

在本研究中，我们描述了一种新的与牛血清白蛋白 (PcSiN3M-BSA) 结合的硅酞菁及其在鼠巨噬细胞 (J774.A1) 中的光动力活性。还研究了非共轭前体，双（三甲基氨基乙氧基）-酞菁硅（IV）（PcSiN3M）。化合物 PcSiN3M 和 PcSiN3M-BSA 在黑暗中没有表现出细胞毒性，但在暴露于 5M 光敏剂和 45 J cm ^-2辐射。这些条件足以分别将用 PcSiN3M 和 PcSiN3M-BSA 处理的细胞的细胞活力降低至 40% 和 5%。这些结果表明，当与 BSA 结合时，PcSiN3M 的光动力活性增加了 87%。这项工作中显示的结果表明 PcSiN3M-BSA 被 J774.A1 细胞吸收更高，这有助于其与未结合形式 PcSiN3N 相比具有更高的光活性。