High-voltage pulsed electric fields (PEF) and pulsed discharge plasmas have received a great deal of attention in the fields of biochemistry, medicine, agriculture and food industry. The destruction phenomenon of cell membranes has been confirmed by PEF and discharge plasma, and it can be used for inactivating microorganisms and extracting cell contents. In recent years, it has been reported that PEF changes the activity of proteins such as enzymes. This review describes how PEF and pulsed discharge plasma function in enzyme activity change and permeabilization in food processing. It is thought that PEF affects the three-dimensional structure of proteins, and research is underway. Here, we will introduce the latest research results on the mechanism of enzyme activity change by PEF. In addition, we will introduce the latest research on changes in cell permeability using shock waves caused by the pulsed discharge plasma.

高压脉冲电场（PEF）和脉冲放电等离子体在生物化学、医学、农业和食品工业领域受到广泛关注。PEF和放电等离子体证实了细胞膜的破坏现象，可用于灭活微生物和提取细胞内容物。近年来，有报道称PEF会改变酶等蛋白质的活性。本综述描述了 PEF 和脉冲放电等离子体在食品加工中酶活性变化和透化中的作用。认为PEF影响蛋白质的三维结构，研究正在进行中。在这里，我们将介绍PEF改变酶活性机制的最新研究成果。此外，