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Efficacy of some native entomopathogenic nematodes against the alfalfa weevil, Hypera postica (Gyllenhal) (Coleoptera: Curculionidae), and the lucerne beetle, Gonioctena fornicata (Brüggemann) (Coleoptera: Chrysomelidae), adults under laboratory conditions
Egyptian Journal of Biological Pest Control ( IF 2.1 ) Pub Date : 2021-06-08 , DOI: 10.1186/s41938-021-00436-z
Ayşegül Çağlayan , Turgut Atay , İlker Kepenekci

Entomopathogenic nematodes (EPNs) have more important role in biological control of economic insect pests. The effect of native EPNs on adults of the lucerne beetle, Gonioctena fornicata (Brüggemann, 1873) (Coleoptera: Chrysomelidae), and the alfalfa weevil, Hypera postica (Gyllenhal, 1813) (Coleoptera: Curculionidae), which are important alfalfa pests in Turkey and around the world, was investigated. Dose-mortality assays were carried out with 5 isolates [Steinernema carpocapsae (Weiser, 1955) (Nematoda: Steinernematidae) (Black sea isolate), S. feltiae Filipjev, 1934 (isolate 09-31), Heterorhabditis bacteriophora Poinar, 1976 (Nematoda: Heterorhabditidae) (isolate 09-43), H. bacteriophora Tokat-Songut, and S. carpocapsae Tokat-Ulas] using doses of 500, 1000, and 2000 IJs ml−1 under the laboratory conditions. Studies showed that all isolates had an effect 90% and more at 2000 IJs ml−1 and at the end of 112 h [except, H. bacteriophora (isolate 09-43) and H. bacteriophora Tokat-Songut isolates against H. postica]. In addition, LT30, LT50, and LT90 values at 1000 IJs ml−1 were determined. According to the results, G. fornicata adults were susceptible to all isolates tested in the study and H. postica adults were susceptible to the isolates S. carpocapsae (Black sea isolate), S. feltiae (isolate 09-31), and S. carpocapsae Tokat-Ulas. This is the first study conducted in Turkey for the virulence of EPNs against G. fornicata and H. postica.

中文翻译:

一些本地昆虫病原线虫在实验室条件下对苜蓿象鼻虫Hypera postica (Gyllenhal) (鞘翅目: Curculionidae) 和苜蓿甲虫Gonioctena fornicata (Brüggemann) (鞘翅目:Chrysomelidae) 的功效

昆虫病原线虫(EPNs)在经济害虫的生物防治中具有更重要的作用。原生 EPN 对苜蓿甲虫 Gonioctena fornicata (Brüggemann, 1873) (Coleoptera: Chrysomelidae) 和苜蓿象鼻虫 Hypera postica (Gyllenhal, 1813) (Coleoptera: Curculionidae) 成虫的影响,它们是土耳其重要的害虫并在世界各地进行了调查。对 5 个分离株 [Steinernema carpocapsae (Weiser, 1955) (Nematoda: Steinernematidae) (Black sea separator), S. Feeliae Filipjev, 1934 (isolate 09-31), Heterorhabditis bacteriophora19da Heterorhabditidae)(隔离 09-43)、H. bacteriophora Tokat-Songut 和 S. carpocapsae Tokat-Ulas] 在实验室条件下使用 500、1000 和 2000 IJs ml-1 的剂量。研究表明,所有分离株在 2000 IJs ml-1 和 112 小时结束时均具有 90% 或更多的效果 [除了 H. bacteriophora(分离株 09-43)和 H. bacteriophora Tokat-Songut 分离株对 H. postica 的作用] . 此外,还确定了 1000 IJs ml-1 时的 LT30、LT50 和 LT90 值。根据结果​​,G. fornicata 成虫对研究中测试的所有分离株易感,而 H. postica 成虫对 S. carpocapsae(黑海分离株)、S. Feeliae(分离株 09-31)和 S. Carpocapsae Tokat-Ulas。这是在土耳其针对 EPNs 对 G. fornicata 和 H. postica 的毒力进行的第一项研究。和 LT90 值在 1000 IJs ml-1 时被确定。根据结果​​,G. fornicata 成虫对研究中测试的所有分离株易感,而 H. postica 成虫对 S. carpocapsae(黑海分离株)、S. Feeliae(分离株 09-31)和 S. Carpocapsae Tokat-Ulas。这是在土耳其进行的第一项 EPNs 对 G. fornicata 和 H. postica 毒力的研究。和 LT90 值在 1000 IJs ml-1 时被确定。根据结果​​,G. fornicata 成虫对本研究中测试的所有分离株均敏感,而 H. postica 成虫对 S. carpocapsae(黑海分离株)、S. Feeliae(分离株 09-31)和 S. Carpocapsae Tokat-Ulas。这是在土耳其进行的第一项 EPNs 对 G. fornicata 和 H. postica 毒力的研究。
更新日期:2021-06-08
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