当前位置: X-MOL 学术Part. Fibre Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Underestimated health risks: polystyrene micro- and nanoplastics jointly induce intestinal barrier dysfunction by ROS-mediated epithelial cell apoptosis
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2021-06-07 , DOI: 10.1186/s12989-021-00414-1
Boxuan Liang 1 , Yizhou Zhong 1 , Yuji Huang 1 , Xi Lin 1 , Jun Liu 1 , Li Lin 1 , Manjiang Hu 1 , Junying Jiang 2 , Mingzhu Dai 3 , Bo Wang 1 , Bingli Zhang 1 , Hao Meng 1 , Jesse Justin J Lelaka 1 , Haixia Sui 4 , Xingfen Yang 5 , Zhenlie Huang 1
Affiliation  

Micro- and nanoplastic pollution has become a global environmental problem. Nanoplastics in the environment are still hard to detect because of analysis technology limitations. It is believed that when microplastics are found in the environment, more undetected nanoplastics are around. The current “microplastic exposure” is in fact the mixture of micro- and nanoplastic exposures. Therefore, the biological interaction between organisms among different sizes of micro- and nanoplastics should not be neglected. We measured the biodistribution of three polystyrene (PS) particles (50 nm PS, PS50; 500 nm PS, PS500; 5000 nm PS, PS5000) under single and co-exposure conditions in mice. We explored the underlying mechanisms by investigating the effects on three major components of the intestinal barrier (the mucus layer, tight junctions and the epithelial cells) in four intestine segments (duodenum, jejunum, ileum and colon) of mice. We found that the amounts of both PS500 and PS5000 increased when they were co-exposed with PS50 for 24 h in the mice. These increased amounts were due primarily to the increased permeability in the mouse intestines. We also confirmed there was a combined toxicity of PS50 and PS500 in the mouse intestines. This manifested as the mixture of PS50 and PS500 causing more severe dysfunction of the intestinal barrier than that caused by PS50 or PS500 alone. We found that the combined toxicity of PS micro- and nanoplastics on intestinal barrier dysfunction was caused primarily by reactive oxygen species (ROS)-mediated epithelial cell apoptosis in the mice. These findings were further confirmed by an oxidants or antioxidants pretreatment study. In addition, the combined toxicity of PS micro- and nanoplastics was also found in the mice after a 28-day repeated dose exposure. There is a combined toxicity of PS50 and PS500 in the mouse intestines, which was caused primarily by ROS-mediated epithelial cell apoptosis in the mice. Considering that most recent studies on PS micro- and nanoplastics have been conducted using a single particle size, the health risks of exposure to PS micro- and nanoplastics on organisms may be underestimated.

中文翻译:

被低估的健康风险:聚苯乙烯微塑料和纳米塑料通过 ROS 介导的上皮细胞凋亡共同诱导肠道屏障功能障碍

微塑料和纳米塑料污染已成为一个全球性的环境问题。由于分析技术的限制,环境中的纳米塑料仍然难以检测。人们相信,当在环境中发现微塑料时,周围就会有更多未被发现的纳米塑料。目前的“微塑料暴露”实际上是微塑料和纳米塑料暴露的混合体。因此,不同尺寸的微塑料和纳米塑料之间生物之间的生物相互作用不容忽视。我们测量了三种聚苯乙烯 (PS) 颗粒 (50 nm PS, PS50; 500 nm PS, PS500; 5000 nm PS, PS5000) 在小鼠单次和共同暴露条件下的生物分布。我们通过研究对肠道屏障三个主要成分(粘液层、小鼠的四个肠段(十二指肠、空肠、回肠和结肠)中的紧密连接和上皮细胞。我们发现,当 PS500 和 PS5000 与 PS50 共同暴露 24 小时后,小鼠体内的 PS500 和 PS5000 的量都会增加。这些增加的数量主要是由于小鼠肠道通透性增加。我们还确认了 PS50 和 PS500 在小鼠肠道中的综合毒性。这表现为 PS50 和 PS500 的混合物比单独使用 PS50 或 PS500 引起的肠道屏障功能障碍更严重。我们发现 PS 微塑料和纳米塑料对肠道屏障功能障碍的联合毒性主要是由活性氧 (ROS) 介导的小鼠上皮细胞凋亡引起的。一项氧化剂或抗氧化剂预处理研究进一步证实了这些发现。此外,在重复剂量暴露 28 天后,在小鼠中也发现了 PS 微塑料和纳米塑料的联合毒性。PS50 和 PS500 在小鼠肠道中存在联合毒性,这主要是由 ROS 介导的小鼠上皮细胞凋亡引起的。考虑到最近对 PS 微塑料和纳米塑料的研究都是使用单一粒径进行的,因此可能低估了接触 PS 微塑料和纳米塑料对生物体的健康风险。这主要是由 ROS 介导的小鼠上皮细胞凋亡引起的。考虑到最近对 PS 微塑料和纳米塑料的研究都是使用单一粒径进行的,因此可能低估了接触 PS 微塑料和纳米塑料对生物体的健康风险。这主要是由 ROS 介导的小鼠上皮细胞凋亡引起的。考虑到最近对 PS 微塑料和纳米塑料的研究都是使用单一粒径进行的,因此可能低估了接触 PS 微塑料和纳米塑料对生物体的健康风险。
更新日期:2021-06-08
down
wechat
bug