Introduction:

Huge amounts of wastewater are generated during food process, and usually contain high nutrient concentration. The present study investigated the feasibility of dairy and poultry wastewaters as growth media for Chlorella sp. T4 cultivation for concomitant nutrient removal and biomass propagation for biofuel production.

Methods:

Microalgae Chlorella sp. T4 was cultivated in poultry and dairy wastewater collected at different stage of wastewater treatment to study the growth, physiological respond, nutrient removal efficiency and biochemical composition.

Results:

The strains showed phycoremediation potential resulting to high nitrogen and phosphorus removal efficiency in dairy and poultry wastewater ranging from 85–95% and 35–93%, respectively. High biomass yield of 1.28 ± 0.1 g L⁻¹ was obtained in poultry wastewater compared to 0.85 ± 0.02 g L⁻¹ obtained in dairy wastewater. The biomass contained significant amounts of lipids (16.2–25.7 % Dry wt.), carbohydrates (20.7–33.1 % Dry wt.), and proteins (24.5–34.6 % Dry wt.), regardless of the wastewater type. The fatty acid analysis revealed that palmitic (16:0), oleic (18:1), and linoleic (18:2) acids were the major fatty acids accumulated by Chlorella sp. T4 when cultivated in poultry and dairy wastewater. Biodiesel properties of lipids extracted from the cell grown in poultry and dairy wastewater complied with most of the international standards by ASTM D6751 and EN 14214.

Conclusion:

The results of this study revealed that Chlorella sp. T4 is a potential candidate for dairy and poultry wastewater treatment, with a significant accumulation of lipid, protein and carbohydrates for use in biofuel production.

Graphical Abstract

中文翻译：

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小球藻同时生产生物质和生物柴油从乳制品和家禽废水中去除养分。从南非淡水溪流中分离出的 T4

介绍：

食品加工过程中会产生大量废水，通常含有高浓度的营养物质。本研究调查了乳制品和家禽废水作为小球藻生长培养基的可行性。用于伴随养分去除和生物燃料生产的生物质繁殖的 T4 培养。

方法：

微藻小球藻sp. 在废水处理不同阶段收集的家禽和乳制品废水中培养T4，以研究其生长、生理反应、养分去除效率和生化成分。

结果：

这些菌株显示出藻类修复潜力，导致乳制品和家禽废水中的氮和磷去除效率分别为 85-95% 和 35-93%。与在乳制品废水中获得的0.85 ± 0.02 g L ^-1相比，在家禽废水中获得了 1.28 ± 0.1 g L ^{-1 的}高生物量产量。无论废水类型如何，生物质都含有大量脂质（16.2–25.7% 干重）、碳水化合物（20.7–33.1% 干重）和蛋白质（24.5–34.6% 干重）。脂肪酸分析表明，棕榈酸 (16:0)、油酸 (18:1) 和亚油酸 (18:2) 是小球藻积累的主要脂肪酸sp. 在家禽和奶制品废水中培养时的 T4。从家禽和乳制品废水中生长的细胞中提取的脂质的生物柴油特性符合 ASTM D6751 和 EN 14214 的大部分国际标准。

结论：

这项研究的结果表明，小球藻属。T4 是乳制品和家禽废水处理的潜在候选物，具有大量用于生物燃料生产的脂质、蛋白质和碳水化合物。

图形概要