Raman spectroscopy is constantly being developed for the detection of early dental caries. The Raman spectrum background can indicate fluorescent compounds in the enamel which appear e.g. in the caries lesion. However, an in-depth analysis of the results of studies regarding sound enamel is necessary to successfully use fluorescence background in the Raman spectrum of teeth, especially in the detection of very early stages of caries. The purpose of this study was to assess the influence of organic compounds in healthy human enamel on the background intensity in Raman spectra. Raman spectroscopy and cone-beam computed tomography confirmed that the enamel was healthy. Scanning electron microscopy analysis indicated that the area of Raman imaging is smooth, without defects and cracks. Raman spectroscopy results were compared with DIAGNOdent pen 2190. The correlation between the spectroscopic results and DIAGNOdent results was evident. Moreover, Raman spectroscopy showed that the additional layer on the enamel surface could not be related to the presence of porphyrins in caries but to organic compounds in plaque or calculus. This conclusion was confirmed by EDS analysis. The results showed that background in Raman spectroscopy may be useful in dental research. However, it should be treated very cautiously as fluorescence can be related to organic compounds which occur in plaque, not in the carious lesion.

拉曼光谱不断被开发用于检测早期龋齿。拉曼光谱背景可以指示牙釉质中出现的荧光化合物，例如出现在龋齿病变中。然而，要成功地在牙齿的拉曼光谱中使用荧光背景，特别是在检测非常早期的龋齿时，必须对有关健全牙釉质的研究结果进行深入分析。本研究的目的是评估健康人牙釉质中的有机化合物对拉曼光谱背景强度的影响。拉曼光谱和锥形束计算机断层扫描证实牙釉质是健康的。扫描电镜分析表明，拉曼成像区域光滑，无缺陷和裂纹。拉曼光谱结果与 DIAGNOdent pen 2190 进行了比较。光谱结果和DIAGNOdent结果之间的相关性很明显。此外，拉曼光谱表明，牙釉质表面的附加层与龋齿中卟啉的存在无关，而是与牙菌斑或牙结石中的有机化合物有关。EDS分析证实了这一结论。结果表明，拉曼光谱的背景可能对牙科研究有用。然而，应该非常谨慎地对待它，因为荧光可能与牙菌斑中出现的有机化合物有关，而不是在龋病灶中。EDS分析证实了这一结论。结果表明，拉曼光谱的背景可能对牙科研究有用。然而，应该非常谨慎地对待它，因为荧光可能与牙菌斑中出现的有机化合物有关，而不是在龋病灶中。EDS分析证实了这一结论。结果表明，拉曼光谱的背景可能对牙科研究有用。然而，应该非常谨慎地对待它，因为荧光可能与牙菌斑中出现的有机化合物有关，而不是在龋病灶中。