ABSTRACT

All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be circumvented by supplementation of known anaerobic growth factors such as nicotinate, sterols and unsaturated fatty acids. Biosynthetic oxygen requirements of yeasts are typically small and, unless extensive precautions are taken to minimize inadvertent entry of trace amounts of oxygen, easily go unnoticed in small-scale laboratory cultivation systems. This paper discusses critical points in the design of anaerobic yeast cultivation experiments in anaerobic chambers and laboratory bioreactors. Serial transfer or continuous cultivation to dilute growth factors present in anaerobically pre-grown inocula, systematic inclusion of control strains and minimizing the impact of oxygen diffusion through tubing are identified as key elements in experimental design. Basic protocols are presented for anaerobic-chamber and bioreactor experiments.

中文翻译：

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生物反应器和厌氧室中酵母厌氧培养的关键参数和程序

摘要

所有已知的兼性发酵酵母的生长都需要分子氧。只有少数酵母物种可以通过补充已知的厌氧生长因子（例如烟酸盐、甾醇和不饱和脂肪酸）来规避这些要求。酵母的生物合成氧气需求通常很小，并且除非采取广泛的预防措施以尽量减少微量氧气的无意进入，否则很容易在小规模实验室培养系统中被忽视。本文讨论了厌氧室和实验室生物反应器中厌氧酵母培养实验设计的关键点。连续转移或连续培养以稀释厌氧预生长接种物中存在的生长因子、系统地纳入对照菌株以及最大限度地减少氧气通过管道扩散的影响被认为是实验设计中的关键要素。提出了厌氧室和生物反应器实验的基本方案。