For preventing transmission of pathogens from broodstock fish to offspring, the non-hardened eggs are routinely disinfected with iodophore solution immediately after fertilization. In the present study, 25–50 mg l^-1 Chloramine T® (active substance: tosylchloramide), 10–50 μl l^-1 Wofasteril® (active substances: acetylhydroperoxide, hydrogen peroxide, and acetic acid) and 10–50 μl l^-1 of a 30% hydrogen peroxide solution were tested for disinfection of non-hardened brown trout, Salmo trutta, eggs in comparison to 10 ml l^-1 of an iodophore solution (Betaisodona®). Five different egg batches were used and the effect of disinfection on the percentage of eyed stage embryos (32 days post fertilization [dpf] at 9° C) and swim-up larvae (42 dpf), on bacteria, and fungus load (in 3 and 32 dpf embryos using different culture broths and qPCR), and on lipid peroxidation and protein carbonylation (in 3 dpf embryos) was investigated. A 20-min treatment of non-hardened eggs with the 4 biocides in abovementioned concentrations had no negative effect on the percentage of eyed stage embryos (92–100% of the control) and normal shaped swim-up larvae (90–100% of the control). All tested treatments including Betaisodona caused oxidative stress to 3 dpf embryos, as they significantly increased the levels of protein carbonyls for 2- to 3-fold in comparison to non-disinfected controls. Thiobarbituric acid-reactive substances did not increase after 10 ml l^-1 Betaisodona and 10 μl l^-1 hydrogen peroxide treatment, but increased by 1.5- to 3-fold after all other treatments. All tested biocides significantly and persistently decreased the load with bacteria in comparison to non-disinfected controls for 25–99% depending on the disinfection treatment and bacteria species. 50 mg l^-1 Chloramine T, 50 μl l^-1 hydrogen peroxide solution, and 50 μl l^-1 Wofasteril had up to 80% higher disinfection efficiency than 10 ml l^-1 Betaisodona for specific species of bacteria. The oomycete Saprolegnia parasitica and the total bacteria load were persistently decreased neither with Chloramine T, Wofasteril, and hydrogen peroxide nor with Betaisodona. It is concluded that hydrogen peroxide, Wofasteril, and Chloramine T are at least similar efficient for disinfection of non-hardened eggs as iodophor solution. They are more economical as the effective concentrations are much lower.

为了防止病原体从亲鱼传播给后代，未硬化的卵在受精后立即用碘伏溶液进行常规消毒。在本研究中，25–50 mg l ^-1 Chloramine T®（活性物质：甲苯磺酰氯胺）、10–50 μl l ^-1 Wofasteril®（活性物质：乙酰过氧化氢、过氧化氢和乙酸）和 10–50 μl l ^-1 30％过氧化氢溶液用于非硬化斑鳟，消毒测试褐鳟，蛋相比至10ml升^-1的iodophore溶液（Betaisodona®） 。使用了五个不同批次的卵，消毒对眼期胚胎（受精后 32 天 [dpf] 在 9°C）和游泳幼虫（42 dpf）、细菌和真菌负荷（在 3和 32 dpf 胚胎使用不同的培养液和 qPCR），并研究了脂质过氧化和蛋白质羰基化（在 3 dpf 胚胎中）。用上述浓度的 4 种杀菌剂处理非硬化卵 20 分钟对眼期胚胎（对照的 92-100%）和正常形状的游泳幼虫（90-100%控制）。包括 Betaisodona 在内的所有测试处理都会对 3 dpf 胚胎造成氧化应激，因为与未消毒的对照相比，它们显着增加了蛋白质羰基化合物的水平 2 到 3 倍。^-1 Betaiisodona 和 10 μl l ^-1过氧化氢处理，但在所有其他处理后增加了 1.5 至 3 倍。根据消毒处理和细菌种类，所有测试的杀菌剂与未消毒的对照相比，显着且持续地降低了 25-99% 的细菌负荷。50 mg l ^-1氯胺T、50 μl l ^-1过氧化氢溶液和50 μl ^-1沃法斯特比10 ml l ^-1 Betaisodona 对特定细菌的消毒效率高达80% 。卵菌Saprolegnia parasitica并且无论是使用氯胺 T、Wofasteril 和过氧化氢，还是使用甜菜碱，总细菌载量都持续降低。得出的结论是，过氧化氢、Wofasteril 和氯胺 T 对非硬化鸡蛋的消毒效率至少与碘伏溶液相似。它们更经济，因为有效浓度要低得多。