Since the first emergence of influenza viruses, they have caused the flu seasonally worldwide. Precise detection of influenza viruses is required to prevent the spreading of the disease. Herein, we developed an optical biosensor using peptide-immobilized nanopillar structures for the label-free detection of influenza viruses. The spin-on-glass nanopillar structures were fabricated by nanoimprint lithography. A sialic acid-mimic peptide, which can specifically bind to hemagglutinin on the surface of the influenza virus, was immobilized onto the nanopillars via polymerized dopamine. The constructed nanopillar sensor enabled us to detect influenza A viruses in the range of 10³–10⁵ plaque-forming units through simple measurements of reflectance. Our findings suggest that biomimetic modification of nanopillar structures can be an alternative method for the immunodiagnosis of influenza viruses.

自流感病毒首次出现以来，它们已在全球范围内引起季节性流感。需要精确检测流感病毒以防止疾病传播。在此，我们开发了一种使用肽固定化纳米柱结构的光学生物传感器，用于流感病毒的无标记检测。旋涂玻璃纳米柱结构是通过纳米压印光刻法制造的。一种唾液酸模拟肽，可以特异性结合流感病毒表面的血凝素，通过聚合多巴胺固定在纳米柱上。^{构建的纳米柱传感器使我们能够检测 10 3} –10 ⁵范围内的甲型流感病毒通过简单的反射率测量来确定斑块形成单位。我们的研究结果表明，纳米柱结构的仿生修饰可以成为流感病毒免疫诊断的替代方法。