Cestrum species are being used as hedge and ornamental plants in Turkey. In this study, a Cestrum elegans plant showing chlorotic spots, rings and reddening was subjected to high throughput sequencing of small RNAs, in order to clarify its etiology. Results suggested the presence of tomato chlorosis virus (ToCV), which was further confirmed by RT-PCR using two specific primers amplifying RNA-dependent RNA polymerase (RdRp) and coat protein (CP) of the virus. When 25 Cestrum samples were tested using CP primers, one symptomatic C. elegans and four symptomless C. nocturnum were infected by ToCV. The obtained sequences shared 83–99.9% nucleotide identity with ToCV isolates available in the GenBank. Phylogenetic relationship among Turkish tomato isolates of ToCV and those available in the GenBank showed that two Cestrum spp. isolates of ToCV were closely related to Turkish tomato isolates, while the other three clustered with isolates from different countries.

Cestrum 物种在土耳其被用作树篱和观赏植物。在这项研究中，对一株出现褪绿斑点、环和变红的Cestrum elegans植物进行了小 RNA 的高通量测序，以阐明其病因。结果表明存在番茄萎黄病病毒 (ToCV)，通过使用两种特异性引物扩增病毒的 RNA 依赖性 RNA 聚合酶 (RdRp) 和外壳蛋白 (CP)，通过 RT-PCR 进一步证实了这一点。当使用 CP 引物测试 25 个 Cestrum 样品时，一种有症状的线虫和四种无症状的C. nocturnum被ToCV感染。获得的序列与 GenBank 中可用的 ToCV 分离株具有 83-99.9% 的核苷酸同一性。ToCV 的土耳其番茄分离株与 GenBank 中可用的分离株之间的系统发育关系表明，两个Cestrum spp. ToCV 的分离株与土耳其番茄分离株密切相关，而其他三个与来自不同国家的分离株聚集在一起。