Abstract—

Hydrogen is a key energy-yielding substrate for microbial processes in the deep biosphere. Fracture water of a deep gold mine in South Africa is a well-known example of the ecosystem based on a hydrogenotrophic sulfate reducer, “Candidatus Desulforudis audaxviator.” The present work reports the cultivation and pure culture isolation of a novel obligate chemolithotroph reducing sulfate during growth on hydrogen or formate. Phylogenetic analysis of the 16S rRNA gene of the strain Tu-874 showed its clusterization with its closest cultured relative, “Candidatus Desulforudis audaxviator.” However, the low 16S rRNA sequence similarity of 88.95% indicates that the organism represents a new taxonomic group above the species level.

中文翻译：

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从秋明热钻孔中分离出一种新型化学石质硫酸盐还原厚壁菌

摘要——

氢是深层生物圈微生物过程的关键产生能量的底物。南非一个深部金矿的压裂水是基于氢营养硫酸盐还原剂“ Candidatus Desulforudis audaxviator”的生态系统的一个著名例子。目前的工作报告了在氢或甲酸盐上生长过程中一种新型专性化学无机盐还原硫酸盐的培养和纯培养分离。菌株 Tu-874 的 16S rRNA 基因的系统发育分析显示其与其最近的培养亲属“ Candidatus Desulforudis audaxviator”聚类。然而，88.95% 的低 16S rRNA 序列相似性表明该生物代表了一个高于物种水平的新分类群。