Abstract

Many microorganisms readily form microbial biofilms whose resistance to antimicrobial compounds is several orders of magnitude higher than that of the planktonic form. One such genus is the yeast Candida, whose species include both human commensals and opportunistic pathogens that are the cause of many fungal infections. Increasing efforts are therefore made to find substances that could replace currently used drugs, or synergistically interact with them and therefore reinforce their action at an acceptable concentration against both cell suspensions and biofilm populations. We investigated the effect of natural-derived compounds chitosan and a commonly used antibiotic amphotericin B against Candida albicans, C. parapsilosis and C. krusei. Chitosan has proven to be a very effective agent against all Candida species both in suspensions and in biofilm formation and eradication. A combination of chitosan and amphotericin B had a strong synergistic effect even at low concentrations. We achieved the reduction of effective concentration of amphotericin B for inhibition of formation and eradication of Candida spp. biofilm, which may be utilized in medicine as an effective approach in lessening the antibiotic concentrations needed for successful treatment.

中文翻译：

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壳聚糖和两性霉素 B 对白色念珠菌、近平滑念珠菌和克柔念珠菌的浮游生物和生物膜种群的协同抑制作用

摘要

许多微生物很容易形成微生物生物膜，其对抗菌化合物的抗性比浮游形式高几个数量级。一种这样的属是酵母念珠菌，其物种包括人类共生菌和引起许多真菌感染的机会性病原体。因此，越来越多的努力寻找可以替代目前使用的药物或与它们协同相互作用的物质，从​​而在可接受的浓度下增强它们对细胞悬浮液和生物膜群体的作用。我们研究了天然衍生的化合物壳聚糖和一种常用的抗生素两性霉素 B 对白色念珠菌、近平滑念珠菌和克柔念珠菌的作用. 壳聚糖已被证明是一种非常有效的抗所有念珠菌属物种，无论是悬浮液还是生物膜形成和根除。即使在低浓度下，壳聚糖和两性霉素 B 的组合也具有很强的协同作用。我们实现了两性霉素 B 有效浓度的降低，以抑制念珠菌的形成和根除。生物膜，可在医学中用作降低成功治疗所需的抗生素浓度的有效方法。