Abstract—

Presence of the cell wall glycopolymer rhamnan was established for members of the genus Curtobacterium (family Microbacteriaceae) by using chemical and NMR spectroscopic methods. The rhamnan with the repeating unit, →2)-α-L-Rhap-(1→3)-α-L-Rhap-(1→, was found in C. flaccumfaciens pv. flaccumfaciens VKM Ас-1923^T, C. flaccumfaciens pv. poinsettiae VKM Ас-1924^T, C. pusillum VKM Ac-2099^T, C. herbarum VKM Ac-2512^T, and Curtobacterium sp. VKM Ac-1811. Each of the studied strains also contained a second, acidic phosphate-free polysaccharide. NMR spectra of these polysaccharides differed, indicating the structural differences. Cell wall hydrolysates of all studied strains were found to contain rhamnose, mannose, glucose, galactose, and glucosamine. Further research will facilitate more detailed characterization of the composition of cell wall glycopolymers in Curtobacterium species, as well as evaluation of their taxonomic significance. The data on the polymer structure will contribute to understanding of the mechanisms of colonization and infection of higher organisms by Curtobacterium species and bacterial interaction in microbial communities and with abiotic environmental objects.

中文翻译：

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Curtobacterium 属放线菌中的细胞壁鼠李聚糖

摘要——

通过使用化学和 NMR 光谱方法，为Curtobacterium属（微杆菌科）成员建立了细胞壁糖聚合物鼠李聚糖的存在。与重复单元中鼠李聚糖，→2）-α-L-鼠李糖p - （1→3）-α-L-鼠李糖p - （1→，在发现C. flaccumfaciens PV。flaccumfaciens VKMАс-1923 ^Ť，C. flaccumfaciens PV。poinsettiae VKMАс-1924 ^Ť，C. pusillum VKM的Ac-2099 ^Ť，C.孢VKM的Ac-2512 ^Ť，和Curtobacterium属sp. VKM Ac-1811。每个研究的菌株还含有第二种酸性无磷酸盐多糖。这些多糖的核磁共振谱不同，表明结构差异。发现所有研究菌株的细胞壁水解产物都含有鼠李糖、甘露糖、葡萄糖、半乳糖和葡糖胺。进一步的研究将有助于更详细地表征Curtobacterium物种中细胞壁糖聚合物的组成，以及评估它们的分类学意义。聚合物结构的数据将有助于了解Curtobacterium物种对高等生物的定殖和感染机制以及微生物群落中的细菌与非生物环境物体的相互作用。