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Isolation and identification of endophytic fungi with screening of promotion growth on mycorrhizal fungi in blueberry
Rhizosphere ( IF 3.4 ) Pub Date : 2021-06-08 , DOI: 10.1016/j.rhisph.2021.100389
Xiaolan Guo , Lanfan Yuan , Muhammad Shakeel , Yaqin Wan , Zejun Song , Delu Wang

Endophytic microorganisms can form a reciprocal symbiosis relationship with plants, and some can improve the survival ability of plants in adversity and promote plant growth and development. In order to further understand the isolation of blueberry endophytic fungi, mycorrhizal fungi that promote the growth of blueberries were screened out. In this paper, blueberry root samples were collected from 5 places in Guizhou Province, and the endophytic fungi were identified by morphological and molecular biological methods. The types of mycorrhizal fungi were determined by the way of back-joining method. Based on the mycorrhizal infection, several kinds of easy culture and energy were selected. A strain that effectively infects blueberry plants and promotes the growth and development of blueberries. The results showed that a total of 995 endophytic fungi were isolated from 5 regions in Guizhou, which were divided into 42 genera, of which Chaetomium spp. and Fusarium spp. were the most dominant, representing 22.91% and 16.18% respectively, followed by Phialocephala spp. (9.54%). Through literature reviewed and tie-back experiments, 9 types of mycorrhizas (Y-037, Y-007, Y-078, Y-017, Y-026, X-065, Y-079, Y-012, Y-005). Among them, Y-037 has the strongest infection intensity and significantly promotes plant growth. This research provides a theoretical basis for proposing a set of efficient and mature blueberry mycorrhizal cultivation technology.



中文翻译:

蓝莓内生真菌的分离鉴定及促进生长的蓝莓菌根真菌筛选

内生微生物可以与植物形成互惠共生关系,有些可以提高植物在逆境中的生存能力,促进植物生长发育。为了进一步了解蓝莓内生真菌的分离情况,筛选出促进蓝莓生长的菌根真菌。本文从贵州省5个地方采集蓝莓根样本,采用形态学和分子生物学方法对内生真菌进行鉴定。采用回接法确定菌根真菌的种类。根据菌根感染情况,选择了几种易培养和能量。一种能有效感染蓝莓植株并促进蓝莓生长发育的菌株。结果表明,从贵州省5个地区共分离出内生真菌995株,共分为42属,其中毛壳菌属(Chaetomium spp. 和镰刀菌属。占主导地位,分别占 22.91% 和 16.18%,其次是 Phialocephala spp。(9.54%)。通过文献回顾和回溯实验,9种菌根(Y-037、Y-007、Y-078、Y-017、Y-026、X-065、Y-079、Y-012、Y-005) . 其中Y-037侵染强度最强,对植物生长有显着促进作用。本研究为提出一套高效成熟的蓝莓菌根栽培技术提供了理论依据。其次是Phialocephala spp。(9.54%)。通过文献回顾和回溯实验,9种菌根(Y-037、Y-007、Y-078、Y-017、Y-026、X-065、Y-079、Y-012、Y-005) . 其中Y-037侵染强度最强,对植物生长有显着促进作用。本研究为提出一套高效成熟的蓝莓菌根栽培技术提供了理论依据。其次是Phialocephala spp。(9.54%)。通过文献回顾和回溯实验,9种菌根(Y-037、Y-007、Y-078、Y-017、Y-026、X-065、Y-079、Y-012、Y-005) . 其中Y-037侵染强度最强,对植物生长有显着促进作用。本研究为提出一套高效成熟的蓝莓菌根栽培技术提供了理论依据。

更新日期:2021-06-14
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