Electrospun porous bone scaffolds are known to imitate the extracellular matrix very well and provide an environment through which the tissue formation is enhanced. Although polymeric scaffolds have a great potential in bone tissue regeneration, their weak bioactivity (bone bonding ability) and mechanical properties have left room for improvement. Therefore, the present study focused on the developing a ternary multifunctional platform composed of polycaprolactone (PCL)/silk fibroin (SF)/Zn-substituted Mg₂SiO₄ nanoparticles for bone tissue regeneration. This study is composed of two connected sections including synthesis and characterization of Mg_(2-x)Zn_xSiO₄, x = 0, 0.5, 1, 1.5, 2 through surfactant-assisted sol-gel technique followed by incorporation of the nanoparticles into PCL/SF hybrid scaffold via electrospinning technique. The weight ratios of polymers and ceramic nanoparticles were optimized to reach desirable textural—porosity, pore size, and fiber diameter—and mechanical properties. Having optimized the ternary scaffold, it was then undergone different physical, chemical, and biological tests in vitro. A precise comparison study between the ternary (PCL/SF/ceramic nanoparticles), binary (PCL/SF), and pure PCL was made to shed light on the effect of each composition on the applicability of ternary scaffold. The overall results confirmed that the Mg₁Zn₁SiO₄ nanoparticles-incorporated PCL/SF scaffold with fluorescence property was the one yielding the highest Young's modulus and desirable textural properties. The ternary scaffold showed improved biological properties making it a promising candidate for further studies towards bone tissue regeneration.

众所周知，电纺多孔骨支架可以很好地模仿细胞外基质，并提供一个环境，通过该环境可以增强组织形成。尽管聚合物支架在骨组织再生方面具有巨大潜力，但其较弱的生物活性（骨结合能力）和机械性能仍有待改进。因此，本研究的重点是开发由聚己内酯（PCL）/丝素蛋白（SF）/锌取代的Mg ₂ SiO ₄纳米颗粒组成的用于骨组织再生的三元多功能平台。本研究由两个相连的部分组成，包括 Mg _{(2- x )} Zn _x SiO _{4 的}合成和表征, x = 0, 0.5, 1, 1.5, 2 通过表面活性剂辅助的溶胶-凝胶技术，然后通过静电纺丝技术将纳米颗粒掺入 PCL/SF 混合支架中。聚合物和陶瓷纳米粒子的重量比经过优化，以达到理想的结构——孔隙率、孔径和纤维直径——和机械性能。在优化了三元支架后，对其进行了不同的体外物理、化学和生物测试。三元（PCL/SF/陶瓷纳米粒子）、二元（PCL/SF）和纯PCL之间的精确比较研究旨在阐明每种成分对三元支架适用性的影响。总体结果证实，Mg ₁ Zn ₁ SiO ₄具有荧光特性的纳米颗粒掺入 PCL/SF 支架是一种产生最高杨氏模量和理想质地特性的支架。三元支架显示出改善的生物学特性，使其成为进一步研究骨组织再生的有希望的候选者。