Abstract

A phytoplankton derived nanoporous biogenic silica (BS) scaffold grafted with polymethacrylate β-estradiol molecularly imprinted polymers (MIP) film (MIP@BS) is reported as a selective platform for the determination of β-estradiol using surfaced-enhanced Raman scattering (SERS). The biogenic silica nanoparticles provided high surface area support for the MIP while serving as an active signal enhancement substrate for SERS. The MIP@BS platform demonstrated an excellent linear response (R² = 0.9996) between 0.1 and 4 ng/mL of β-estradiol standards. The limit of detection (LOD) for the MIP@BS SERS sensor for β-estradiol was 0.073 ng/mL. The MIP@BS SERS sensors were successfully evaluated for the determination of β-estradiol in milk, while demonstrating good stability and reusability.

摘要

据报道，一种用聚甲基丙烯酸酯β-雌二醇分子印迹聚合物（MIP@BS）接枝的浮游植物衍生的纳米多孔生物二氧化硅（BS）支架作为表面增强拉曼散射（SERS）测定β-雌二醇的选择性平台. 生物源二氧化硅纳米粒子为 MIP 提供了高表面积支持，同时作为 SERS 的活性信号增强底物。MIP@BS 平台在 0.1 和 4 ng/mL β-雌二醇标准品之间表现出出色的线性响应 (R ^{2 = 0.9996)。}MIP@BS SERS 传感器对 β-雌二醇的检测限 (LOD) 为 0.073 ng/mL。MIP@BS SERS 传感器已成功评估用于测定牛奶中的 β-雌二醇，同时表现出良好的稳定性和可重复使用性。