Abstract

Here, Fe₃O₄ @SiO₂ @Poly (DEMA -EGDMA-CO-VPBA) nanoparticles were prepared as a pH-sensitive nanocomposite for adsorption and controlled release of glycoproteins. The average size of particles was found to be 62.5 nm. The ability of these particles to isolate and enrich the glycoprotein Horseradish Peroxidase and nonglycoprotein of Bovine serum was investigated using analytical and spectroscopic methods. Accordingly, the maximum adsorption capacity of nanocomposite for Horseradish Peroxidase was about 129.8 mgg⁻¹ under optimum pH of 9.3. High performance rate, low concentration needed for glycoprotein separation and enrichment and reusable up to five times, are other features of these particles.

摘要

在这里，Fe ₃ O ₄ @SiO ₂ @Poly (DEMA -EGDMA-CO-VPBA) 纳米颗粒被制备为一种 pH 敏感的纳米复合材料，用于吸附和控制糖蛋白的释放。发现颗粒的平均尺寸为 62.5 nm。使用分析和光谱方法研究了这些颗粒分离和富集牛血清中糖蛋白辣根过氧化物酶和非糖蛋白的能力。因此，在最适pH 9.3 下，纳米复合材料对辣根过氧化物酶的最大吸附容量约为129.8 mgg ^{-1 。}这些颗粒的其他特点是高效率、糖蛋白分离和富集所需的低浓度以及可重复使用多达五次。