ABSTRACT

Green synthesis of selenium nanoparticles (Se-NPs) was done using Abelmoschus esculentus extract as the capping and reducing agent and sodium selenite (Na₂SeO₃) as the selenium source. UV-Vis spectrum confirmed the formation of Se-NPs in the solution. According to the XRD pattern, the average particle size was estimated to be about 30.7 nm. The TEM images shown the spherical shape of Se-NPs. The antimycobacterial effects of Se-NPs were tested against the standard strains of Mycobacterium tuberculosis (H37RV) and Mycobacterium simiae isolates. Antibacterial impacts were examined through the usage of resazurin microtiter assay (REMA) against bacterial strains, including Staphylococcus aureus, Streptococcus mutans, Escherichia coli, and Pseudomonas aeruginosa. There were no significant abilities to be reported from the Se-NPs for combating M. tuberculosis H37Rv and M. simiae strains. Based on the results, the nanoparticles displayed stronger antibacterial activities towards the Gram-positive bacteria.

摘要

以南芥提取物为封端和还原剂，亚硒酸钠（Na ₂ SeO _{3 ）绿色合成硒纳米颗粒（Se-NPs）。}) 作为硒源。UV-Vis 光谱证实了溶液中 Se-NPs 的形成。根据 XRD 图案，估计平均粒径约为 30.7 nm。TEM图像显示了Se-NPs的球形。针对结核分枝杆菌 (H37RV) 和模拟分枝杆菌分离株的标准菌株测试了 Se-NPs 的抗分枝杆菌作用。通过使用刃天青微量滴定法 (REMA) 对细菌菌株（包括金黄色葡萄球菌、变形链球菌、大肠杆菌和铜绿假单胞菌）的抗菌作用进行了检查。没有报道 Se-NPs 对抗 M. tuberculosis H37Rv 和 M. simiae 菌株的显着能力。根据结果​​，纳米颗粒对革兰氏阳性菌表现出更强的抗菌活性。