This study aimed to evaluate methane productivity of Napier grass mono-digestion in single-stage anaerobic completely stirred tank reactors (CSTR) inoculated differently by cow manure (COM), anaerobic sludge (ANS), and a mix of COM and ANS for CSTR1 to 3, respectively. The different inoculation promoted operational performance, stability, and organic loading rate (OLR) differently. The produced biogas and methane varied significantly with OLR (p < 0.05). The maximum gas productivities between 0.42–0.48 L-biogas/g VS_added and 0.27–0.34 L-CH₄/g VS_added were obtained at OLR of 1.0–1.5 g VS/L day. Later, the increase in OLR to 2.0 and 2.4 g VS/L day decreased biogas and methane yields by 24.85–39.65% and 11.66–52.89%. Co-inoculation of COM and ANS was a promising strategy that encouraged more stability in CSTR3 than the other reactors. Consequently, the methanogenic performance was developed in all digesters, depicted clearly by high methane content between 52.94 and 59.30% in produced biogas. A similar microbial structure was found in each CSTR-dominated predominantly by Proteobacteria, Bacteroidetes, and Formicates. About 0.5–3.0% of the whole populations were archaea identified distinctly to Methanocorpusculum, Methanospirillum, and Methanosaeta. This finding confirmed the importance of seed inoculation to practical gas productivity and confined organic loading capacity of Napier grass biomethanation in single-stage wet and mono-substrate digestion processes.

本研究旨在评估牛粪 (COM)、厌氧污泥 (ANS) 以及 CSTR1 的 COM 和 ANS 混合物不同接种的单级厌氧完全搅拌釜反应器 (CSTR) 中纳皮尔草单消化的甲烷生产率。 3、分别。不同的接种对操作性能、稳定性和有机负载率 (OLR) 的促进作用不同。产生的沼气和甲烷随 OLR 变化显着（p  < 0.05）。在 0.42–0.48 L-沼气/g VS_添加和 0.27–0.34 L-CH ₄ /g VS_添加之间的最大产气量在 1.0–1.5 g VS/L 天的 OLR 下获得。后来，OLR 增加到 2.0 和 2.4 g VS/L 天使沼气和甲烷产量降低了 24.85-39.65% 和 11.66-52.89%。COM 和 ANS 的共同接种是一种很有前途的策略，它鼓励 CSTR3 比其他反应器具有更高的稳定性。因此，所有沼气池的产甲烷性能都得到了发展，生产的沼气中甲烷含量在 52.94% 和 59.30% 之间，这清楚地表明了这一点。在每个 CSTR 中发现了类似的微生物结构，主要由Proteobacteria、Bacteroidetes和Formicates 主导。大约 0.5-3.0% 的整个种群是古细菌，可清楚地识别出甲烷菌、甲烷螺菌和甲烷菌。这一发现证实了种子接种对单级湿法和单底物消化过程中纳皮尔草生物甲烷化的实际气体生产率和受限有机负载能力的重要性。