Characterization of the biocontrol activity of three bacterial isolates against the phytopathogen Erwinia amylovora,MicrobiologyOpen

当前位置： X-MOL 学术 › Microbiologyopen › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Characterization of the biocontrol activity of three bacterial isolates against the phytopathogen Erwinia amylovora
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-06-07 , DOI: 10.1002/mbo3.1202
Fadi Dagher ₁ , Arvin Nickzad ₁ , Jie Zheng ₂ , Maria Hoffmann ₂ , Eric Déziel ₁

Affiliation

Antibiotics are sprayed on apple and pear orchards to control, among other pathogens, the bacterium Erwinia amylovora, the causative agent of fire blight. As with many other pathogens, we observe the emergence of antibiotic-resistant strains of E. amylovora. Consequently, growers are looking for alternative solutions to combat fire blight. To find alternatives to antibiotics against this pathogen, we have previously isolated three bacterial strains with antagonistic and extracellular activity against E. amylovora, both in vitro and in planta, corresponding to three different bacterial genera: Here, we identified the inhibitory mode of action of each of the three isolates against E. amylovora. Isolate Bacillus amyloliquefaciens subsp. plantarum (now B. velezensis) FL50S produces several secondary metabolites including surfactins, iturins, and fengycins. Specifically, we identified oxydifficidin as the most active against E. amylovora S435. Pseudomonas poae FL10F produces an active extracellular compound against E. amylovora S435 that can be attributed to white-line-inducing principle (WLIP), a cyclic lipopeptide belonging to the viscosin subfamily (massetolide E, F, L, or viscosin). Pantoea agglomerans NY60 has a direct cell-to-cell antagonistic effect against E. amylovora S435. By screening mutants of this strain generated by random transposon insertion with decreased antagonist activity against strain S435, we identified several defective transposants. Of particular interest was a mutant in a gene coding for a Major Facilitator Superfamily (MFS) transporter corresponding to a transmembrane protein predicted to be involved in the extracytoplasmic localization of griseoluteic acid, an intermediate in the biosynthesis of the broad-spectrum phenazine antibiotic D-alanylgriseoluteic acid.

中文翻译：

三种细菌分离株对植物病原体火梨欧文氏菌的生物防治活性的表征

在苹果园和梨园喷洒抗生素，以控制其他病原体，其中包括火疫病的病原体火疫病菌。与许多其他病原体一样，我们观察到抗生素耐药菌株的出现。梨病菌。因此，种植者正在寻找替代解决方案来对抗火疫病。为了找到针对这种病原体的抗生素替代品，我们之前分离出了三种对大肠杆菌具有拮抗和细胞外活性的细菌菌株。梨病菌，在体外和植物中，对应于三个不同的细菌属：在这里，我们确定了三种分离株中的每一种对大肠杆菌的抑制作用模式。梨病菌。分离解淀粉芽孢杆菌亚种。 plantarum （现在的B.velezensis ）FL50S 产生多种次级代谢产物，包括表面活性素、伊图林和风霉素。具体来说，我们确定氧化双歧定是对抗大肠杆菌最有效的。梨病菌S435。早熟假单胞菌FL10F产生一种针对大肠杆菌的活性细胞外化合物。 amylovora S435 可归因于白线诱导原理 (WLIP)，一种属于粘素亚家族（马赛托内酯 E、F、L 或粘素）的环状脂肽。 Pantoea agglomerans NY60 对大肠杆菌具有直接的细胞间拮抗作用。梨病菌S435。通过筛选由随机转座子插入产生的该菌株的突变体，其针对菌株 S435 的拮抗剂活性降低，我们鉴定了几个有缺陷的转座子。特别令人感兴趣的是编码主要促进子超家族（MFS）转运蛋白的基因突变体，该突变体对应于跨膜蛋白，预计参与灰黄酸的胞质外定位，灰黄酸是广谱吩嗪抗生素D生物合成的中间体 -丙氨酰灰黄酸。

更新日期：2021-06-07

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11