Synergistic Enhancement of Fluorescence and Magnetic Resonance Signals Assisted by Albumin Aggregate for Dual-Modal Imaging,ACS Nano

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Synergistic Enhancement of Fluorescence and Magnetic Resonance Signals Assisted by Albumin Aggregate for Dual-Modal Imaging
ACS Nano ( IF 15.8 ) Pub Date : 2021-06-07 , DOI: 10.1021/acsnano.1c01251
Lirong Wang ₁ , Qing Wan ₁ , Rongyuan Zhang ₂ , Bo Situ ₃ , Kaiyuan Ni ₄ , Jinhao Gao ₅ , Xing Feng ₆ , Pengfei Zhang ₇ , Zhiming Wang ₁ , Anjun Qin ₁ , Ben Zhong Tang _{1,

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Affiliation

State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, AIE Institute, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China.
Department of Urology, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou 215006, China.
Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, United States.
Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
School of Material and Energy, Guangdong University of Technology, Guangzhou 510006, China.
Guangdong Key Laboratory of Nanomedicine, Shenzhen Engineering Laboratory of Nanomedicine and Nanoformulations, CAS Key Laboratory of Health Informatics, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Shenzhen Institute of Molecular Aggregate Science and Engineering, School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, 2001 Longxiang Boulevard, Longgang District, Shenzhen City, Guangdong 518172, China.

Dual-modal fluorescence and magnetic resonance imaging (FLI/MRI) is important for the early diagnosis of malignant tumors. However, facile and opportune strategies to synergistically enhance fluorescence intensity and magnetic resonance (MR) contrast have rarely been reported. Herein, we present a facile strategy using albumin aggregates (AAs) to synergistically enhance the fluorescence intensity by aggregation-induced emission (AIE) and MR contrast with prolonged rotational correlation time (τ_R) of Gd(III) chelates and the diffusion correlation time (τ_D) of surrounding water molecules. The amphiphilic dual-modal FLI/MRI probe of NGd was facilely loaded into albumin pockets and then formed AAs to generate a supramolecular structure of NGd-albumin aggregates (NGd-AAs), which show excellent biocompatibility and biosafety, and exhibit superior fluorescence quantum yield and r₁ over NGd with 6- and 8-fold enhancement, respectively. Moreover, compared with the clinical MRI contrast agent Gd-DOTA, r₁ of NGd-AAs showed a 17-fold enhancement. Therefore, NGd-AAs successfully elicited high-performance dual-modal FLI/MRI in vitro and in vivo and high contrast MR signals were observed in the liver and tumor after intravenous injection of NGd-AAs at a dosage of 6 μmol Gd(III)/kg body weight. This generic and feasible strategy successfully realized a synergistic effect for dual-modal FLI/MRI.

中文翻译：

用于双模态成像的白蛋白聚集体辅助荧光和磁共振信号的协同增强

双模态荧光和磁共振成像（FLI/MRI）对于恶性肿瘤的早期诊断非常重要。然而，很少报道协同增强荧光强度和磁共振 (MR) 对比度的简便和适当的策略。在此，我们提出了一种使用白蛋白聚集体 (AAs) 的简便策略，通过聚集诱导发射 (AIE) 和 MR 对比度与Gd(III) 螯合物的延长旋转相关时间 (τ _R ) 和扩散相关时间协同增强荧光强度（τ _d) 周围的水分子。NGd 的两亲性双模态 FLI/MRI 探针被轻松加载到白蛋白袋中，然后形成 AA 以生成 NGd-白蛋白聚集体 (NGd-AAs) 的超分子结构，具有优异的生物相容性和生物安全性，并表现出优异的荧光量子产率和r ₁在 NGd 上分别具有 6 倍和 8 倍的增强。此外，与临床 MRI 造影剂 Gd-DOTA 相比，NGd-AAs 的r ₁显示出 17 倍的增强。因此，NGd-AAs在体外和体内成功地引发了高性能的双模态 FLI/MRI在以 6 μmol Gd(III)/kg 体重的剂量静脉注射 NGd-AAs 后，在肝脏和肿瘤中观察到高对比度 MR 信号。这种通用且可行的策略成功实现了双模态 FLI/MRI 的协同效应。

更新日期：2021-06-22

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