The aim of this study was to explore the effects of the potential of vitamin C in relieving lead-induced antioxidant and immune responses in Grass Carp Ctenopharyngodon idella kidney (CIK) cells. In this study, CIK cells were treated with vitamin C (0, 60, 80, 100, 120, and 140 µM) and lead (0 and 25 µM). Cell viability, oxidative response, and immune-related gene expression were assessed. These results showed that cell viability was significantly decreased by exposure to lead and that vitamin C supplementation alone had no significant effect on cell viability, but vitamin C supplementation can alleviate the effect of lead on cell viability. Furthermore, vitamin C supplementation can increase the antioxidant capacity and the expression of immune-related genes. Ultimately, these results suggest that vitamin C has a protective effect on CIK cells when they are exposed to lead.

中文翻译：

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维生素 C 对草鱼肾细胞中铅诱导的细胞活力水平、氧化应激和免疫相关基因表达的影响

本研究的目的是探索维生素 C 的潜力在缓解草鱼Ctenopharyngodon idella铅诱导的抗氧化和免疫反应中的作用。肾 (CIK) 细胞。在这项研究中，CIK 细胞用维生素 C（0、60、80、100、120 和 140 µM）和铅（0 和 25 µM）处理。评估细胞活力、氧化反应和免疫相关基因表达。这些结果表明，接触铅会显着降低细胞活力，单独补充维生素 C 对细胞活力没有显着影响，但补充维生素 C 可以减轻铅对细胞活力的影响。此外，补充维生素 C 可以增加抗氧化能力和免疫相关基因的表达。最终，这些结果表明，当 CIK 细胞暴露于铅时，维生素 C 对它们具有保护作用。