Biomodulation of biopolymeric materials for human healthcare is an active and promising area of research. In the present study, chitosan nanoparticles grafted fish gelatin bio-nanocomposite membranes were prepared and their physical and structural properties were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The particle size of the chitosan nanoparticles (Chi-NPs) was in the range of 83–193 nm. The porosity of bio-nanocomposite membranes was in the range of 50–58%. The swelling and equilibrium water content of composite membranes increased significantly. The microstructures of bio-nanocomposite membranes were well-defined with relatively smooth surface, interconnected pores and voids. The cytotoxicity of bio-nanocomposite membranes was investigated using L929 fibroblast cells, and results showed that the membranes were non-toxic and their cell viability percentages were substantially higher as compared to control. Implantation of these membranes was conducted by animal experimental studies, and the results confirmed that bio-nanocomposite membranes have the potential to embrace safety, cell promotion and proliferation. Histopathological examination suggested that the bio-nanocomposite membrane was completely absorbed and it had an ability to promote the formation of granulation tissue and enhance epithelialization. The results of the present investigation demonstrated that chitosan nanoparticles grafted fish gelatin bio-nanocomposite membranes have good potential for biomedical and tissue engineering applications.

用于人类保健的生物聚合物材料的生物调节是一个活跃且有前途的研究领域。在本研究中，制备了壳聚糖纳米粒子接枝鱼明胶生物纳米复合膜，并使用傅里叶变换红外光谱 (FTIR)、扫描电子显微镜 (SEM) 和原子力显微镜 (AFM) 对其物理和结构特性进行了表征。壳聚糖纳米颗粒（Chi-NPs）的粒径在 83-193 nm 范围内。生物纳米复合膜的孔隙率在 50-58% 的范围内。复合膜的溶胀和平衡水含量显着增加。生物纳米复合膜的微观结构具有相对光滑的表面、相互连接的孔隙和空隙。生物纳米复合膜的细胞毒性研究L929成纤维细胞，结果表明膜是无毒的，与对照相比，它们的细胞活力百分比显着更高。这些膜的植入是通过动物实验研究进行的，结果证实生物纳米复合膜具有安全性、细胞促进和增殖的潜力。组织病理学检查表明，生物纳米复合膜被完全吸收，具有促进肉芽组织形成和增强上皮化的能力。本研究的结果表明，壳聚糖纳米粒子接枝鱼明胶生物纳米复合膜具有良好的生物医学和组织工程应用潜力。