Advancements in liquid chromatography and mass spectrometry over the last decades have led to a significant development in mass spectrometry-based proteome quantification approaches. An increasingly attractive strategy is multiplex isotope labeling, which significantly improves the accuracy, precision and throughput of quantitative proteomics in the data-dependent acquisition mode. Isotope labeling-based approaches can be classified into MS1-based and MS2-based quantification. In this review, we give an overview of approaches based on chemical isotope labeling and discuss their principles, benefits, and limitations with the goal to give insights into fundamental questions and provide a useful reference for choosing a method for quantitative proteomics. As a perspective, we discuss the current possibilities and limitations of multiplex, isotope labeling approaches for the data-independent acquisition mode, which is increasing in popularity.

中文翻译：

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用于定量蛋白质组学的化学同位素标记

过去几十年液相色谱和质谱的进步导致了基于质谱的蛋白质组量化方法的重大发展。一个越来越有吸引力的策略是多重同位素标记，它显着提高了数据依赖采集模式下定量蛋白质组学的准确性、精确度和通量。基于同位素标记的方法可分为基于 MS1 和基于 MS2 的量化。在这篇综述中，我们概述了基于化学同位素标记的方法，并讨论了它们的原理、优点和局限性，目的是深入了解基本问题，并为选择定量蛋白质组学方法提供有用的参考。从一个角度来看，我们讨论了当前多路复用的可能性和局限性，